高效液相色谱-质谱法测定黑果腺肋花楸酒中酚类物质  

Determination of Phenolic Substances in Aronia Melanocarpa Wine by HPLC-Tandem Mass Spectrometry

作　　者：郭金颖 赵冬梅 GUO Jin-ying;ZHAO Dong-mei(Qinhuangdao Food and Drug Inspection Center,Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市食品药品检验中心,河北秦皇岛066000

出　　处：《饮料工业》2024年第4期24-28,共5页Beverage Industry

基　　金：河北省市场监督管理局计划项目(2021ZD42)。

摘　　要：建立了黑果腺肋花楸酒中6种酚类物质的高效液相色谱-质谱测定方法,样品经10%乙醇稀释后使用高效液相色谱-串联质谱仪进行检测,在正离子多反应监测模式下采集,外标法定量。结果表明,绿原酸、表儿茶素、槲皮素、芦丁、白藜芦醇、矢车菊素6种酚类物质在10.00-500.00ng/mL浓度范围内线性关系良好,相关系数R^(2)均大于0.999,定量限均为10ng/mL;每种物质的低、中、高3个浓度水平的加标回收率在84.7%~98.0%之间,相对标准偏差在1.1%~6.7%之间。该方法前处理简便、检测结果准确、可靠,可用于花楸酒中酚类物质的同时检测。In this study,a HPLC-mass spectrometry method was established for the detection of 6 bioactive phenolic substances in Aronia melanocarpa wine.The method conditions were:as follows:ESI source positive ion mode,ion spray voltage 5000V,ion source temperature 550℃,gas curtain gas pressure 45 psi,and detection time 10 min.Results:The R^(2) of the curves of 6 phenolic substances was greater than 0.999.The recoveries of three levels of each substance ranged from 84.7%to 98.0%,the relative standard deviations ranged from 1.1%to 6.7%,and the limits of quantification were 10ng/mL.Conclusion:The method is simple,accurate and reliable,and can be used for simultaneous detection of bioactive phenols in Aronia melanocarpa wine.

关 键 词：黑果腺肋花楸酒 酚类物质 高效液相色谱-质谱 

分 类 号：TS207[轻工技术与工程—食品科学]