Functionalized TiCu/TiCuN coating promotes osteoporotic fracture healing by upregulating the Wnt/β-catenin pathway  

作　　者：Jia Tan Ling Ren Kai Xie Lei Wang Wenbo Jiang Yu Guo Yongqiang Hao 

机构地区：[1]Department of Orthopaedic Surgery,Shanghai Key Laboratory of Orthopaedic Implants,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China [2]Clinical and Translational Research Center for 3D Printing Technology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China [3]Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110000,China [4]Musculoskeletal Tumor Center,Peking University People’s Hospital,Beijing 100044,China

出　　处：《Regenerative Biomaterials》2023年第1期126-139,共14页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China[81972058 and 81902194];the Science and Technology Commission of Shanghai Municipality[22YF1422900 and 21002411200];the Shanghai Municipal Key Clinical Specialty,China[shslczdzk06701];Huangpu District Industrial Support Fund[XK2020009];the National Facility for Translational Medicine(Shanghai),China[TMSZ-2020-207];the Shanghai Engineering Research Center of Orthopedic Innovative Instruments and Personalized Medicine Instruments and Personalized Medicine[19DZ2250200].

摘　　要：Osteoporosis results in decreased bone mass and insufficient osteogenic function.Existing titanium alloy implants have insufficient osteoinductivity and delayed/incomplete fracture union can occur when used to treat osteoporotic fractures.Copper ions have good osteogenic activity,but their dose-dependent cytotoxicity limits their clinical use for bone implants.In this study,titanium alloy implants functionalized with a TiCu/TiCuN coating by arc ion plating achieved a controlled release of copper ions in vitro for 28 days.The coated alloy was co-cultured with bone marrow mesenchymal stem cells and showed excellent biocompatibility and osteoinductivity in vitro.A further exploration of the underlying mechanism by quantitative real-time polymerase chain reaction and western blotting revealed that the enhancement effects are related to the upregulation of genes and proteins(such as axin2,β-catenin,GSK-3β,p-GSK-3β,LEF1 and TCF1/TCF7)involved in the Wnt/β-catenin pathway.In vivo experiments showed that the TiCu/TiCuN coating significantly promoted osteoporotic fracture healing in a rat femur fracture model,and has good in vivo biocompatibility based on various staining results.Our study confirmed that TiCu/TiCuN-coated Ti promotes osteoporotic fracture healing associated with the Wnt pathway.Because the coating effectively accelerates the healing of osteoporotic fractures and improves bone quality,it has significant clinical application prospects.

关 键 词：nitride-titanium-copper orthopaedic implant OSTEOGENESIS OSTEOINTEGRATION osteoporotic fractures 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]