3D-printed GelMA/CaSiO_(3)composite hydrogel scaffold for vascularized adipose tissue restoration  

作　　者：Jupei Zhang Zhen Zeng Yanxin Chen Li Deng Yanxin Zhang Yumei Que Yiren Jiao Jiang Chang Zhihong Dong Chen Yang 

机构地区：[1]School of Mechanical Engineering,Chengdu University,Chengdu 610106,China [2]Joint Centre of Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China [3]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China [4]College of Materials Science and Opto-electronic Technology,University of Chinese Academy of Sciences,Beijing 100049,China [5]Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China

出　　处：《Regenerative Biomaterials》2023年第1期1073-1084,共12页再生生物材料（英文版）

基　　金：supported by National Natural Science Foundation of China(32271386,31900945);Zhejiang Traditional Chinese Medicine Scientific Research Fund Project(2022ZB342);Chengdu Municipal Technological Innovation R&D Project(2021-YF05-01871-SN);Project of Chengdu Municipal Health Commission(2021059);the seed grants from the Wenzhou Institute,University of Chinese Academy of Sciences(WIUCASQD2020013,WIUCASQD2021030);the funding from First Affiliated Hospital of Wenzhou Medical University.

摘　　要：The increased number of mastectomies,combined with rising patient expectations for cosmetic and psychosocial outcomes,has necessitated the use of adipose tissue restoration techniques.However,the therapeutic effect of current clinical strategies is not satisfying due to the high demand of personalized customization and the timely vascularization in the process of adipose regeneration.Here,a composite hydrogel scaffold was prepared by three-dimensional(3D)printing technology,applying gelatin methacrylate anhydride(GelMA)as printing ink and calcium silicate(CS)bioceramic as an active ingredient for breast adipose tissue regeneration.The in vitro experiments showed that the composite hydrogel scaffolds could not only be customized with controllable architectures,but also significantly stimulated both 3T3-L1 preadipocytes and human umbilical vein endothelial cells in multiple cell behaviors,including cell adhesion,proliferation,migration and differentiation.Moreover,the composite scaffold promoted vascularized adipose tissue restoration under the skin of nude mice in vivo.These findings suggest that 3D-printed GelMA/CS composite scaffolds might be a good candidate for adipose tissue engineering.

关 键 词：3D-printing BIOCERAMIC composite scaffold ADIPOGENESIS ANGIOGENESIS 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术] R318.08[自动化与计算机技术—计算机科学与技术] TQ427.26[医药卫生—生物医学工程]