艾叶黄酮的超声辅助离子液体提取及抗氧化、降血糖活性  

Ultrasound-assisted ionic liquid extraction,antioxidant and hypoglycemic activities of Artemisiae argyi leaves flavonoids

作　　者：赫丁轩 刁婷婷 张少军 李卓佳 王海燕[1] 陈琼[1,2] HE Dingxuan;DIAO Tingting;ZHANG Shaojun;LI Zhuojia;WANG Haiyan;CHEN Qiong(School of Pharmacy,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China;Henan Dabie Mountain Argyi Comprehensive Development and Utilization Engineering Technology Research Center,Xinyang 464000,Henan,China)

机构地区：[1]信阳农林学院药学院,河南信阳464000 [2]河南省大别山艾草综合开发利用工程技术研究中心,河南信阳464000

出　　处：《精细化工》2024年第9期1978-1986,共9页Fine Chemicals

基　　金：河南省科技攻关计划项目(242102230094,242102310575,242102110243);河南省重大专项创新示范项目(201111310900)。

摘　　要：采用单因素实验及响应面分析法对超声辅助离子液体提取艾叶黄酮工艺进行了优化,对艾叶黄酮体外抗氧化能力和降血糖活性进行了测试。结果表明,在以质量浓度为8 g/L的1-丁基-3-甲基咪唑溴盐([C4mim]Br)乙醇水溶液为提取溶剂、料液比(g∶mL)为1∶30、超声时间68 min的最佳提取工艺条件下,艾叶黄酮的提取率为14.36%。艾叶黄酮对1,1-二苯基-2-三硝基苯肼自由基(DPPH•)、2,2'-联氮-二(3-乙基苯并噻唑-6-磺酸)二铵盐自由基(ABTS^(+)•)和•OH具有较好的清除能力,对α-淀粉酶和α-葡萄糖苷酶具有较好的活性抑制能力,表现出一定的抗氧化性和降血糖功效。质量浓度为0.5 g/L艾叶黄酮对DPPH•和ABTS^(+)•的清除率分别为81.05%和93.81%,半数抑制浓度(IC_(50))分别为0.15、0.12 g/L;质量浓度1.0 g/L艾叶黄酮对•OH的清除率为70.28%,IC_(50)为0.60 g/L;质量浓度1.0 g/L艾叶黄酮对α-淀粉酶的抑制率为64.70%,IC_(50)为0.59 g/L;质量浓度0.5 g/L艾叶黄酮对α-葡萄糖苷酶的抑制率为56.68%,IC_(50)为0.37 g/L。The extraction process of flavonoids from Artemisia argyi leaves with ultrasound-assisted ionic liquid was optimized via single factor experiments and response surface analysis,and Artemisia argyi leaves flavonoids obtained was further analyzed for their in vitro antioxidant and hypoglycemic activities.The results showed that under the optimal extraction process of 1-butyl-3-methylimidazolium bromide([C4mim]Br)ethanol water solution with a mass concentration 8 g/L as extraction solvent,material-liquid ratio(g∶mL)of 1∶30 and ultrasonic time of 68 min,the extraction rate of Artemisia argyi leaves flavonoids was 14.36%.Artemisia argyi leaves flavonoids exhibited good scavenging abilities against 1,1-diphenyl-2-trinitrophenylhydrazine radicals(DPPH•),2,2'-diazo-di(3-ethylbenzothiazole-6-sulfonic acid)diamine salts free radicals(ABTS^(+)•)and•OH,and good inhibition activity againstα-amylase andα-glucosidase,indicating certain antioxidant and hypoglycemic activities.The clearance rates of Artemisia argyi leaves flavonoids with a mass concentration of 0.5 g/L on DPPH•and ABTS^(+)•were 81.05%and 93.81%,and the median inhibitory concentration(IC_(50))were 0.15 and 0.12 g/L,respectively.The clearance rate of•OH at Artemisia argyi leaves flavonoids with a mass concentration of 1.0 g/L was 70.28%,and IC_(50)was 0.60 g/L.The inhibitory rate ofα-amylase was 64.70%and IC_(50)was 0.59 g/L at Artemisia argyi leaves flavonoids with a mass concentration of 1.0 g/L.The inhibitory rate ofα-glucosidase was 56.68%and IC_(50)was 0.37 g/L at Artemisia argyi leaves flavonoids with a mass concentration of 0.5 g/L.

关 键 词：艾叶 黄酮 离子液体 抗氧化性 降血糖活性 中药现代化技术 

分 类 号：R284.2[医药卫生—中药学]