酶促酯交换反应中金属有机框架固定化脂肪酶研究进展  

Research progress of lipase immobilization with metal-organic frameworks for the enzymatic transesterification

作　　者：唐晨 丛文杰 张建国[1] 王明轩 李振海 王继国 TANG Chen;CONG Wenjie;ZHANG Jianguo;WANG Mingxuan;LI Zhenhai;WANG Jiguo(School of Health Science&Engineering,University of Shanghai for Science&Technology,Shanghai 200093,China;Toroivd Technology Co.Ltd.,Shanghai 200439,China)

机构地区：[1]上海理工大学,健康科学与工程学院,上海200093 [2]天筛(上海)科技有限公司,上海200439

出　　处：《食品与发酵工业》2024年第17期363-370,共8页Food and Fermentation Industries

基　　金：中国博士后科学基金第71批面上资助项目(2022M712140);上海市“超级博士后”激励计划项目(2022472);上海市国际科技合作基金项目(19230742900)。

摘　　要：酶促油脂转化为脂肪酸酯能同时满足食品安全和“双碳”战略的需求,然而游离脂肪酶性质脆弱、回收难、成本高等弊端限制了其大规模工业化应用。利用多孔材料为载体固定化脂肪酶能够克服上述问题,合理设计载体结构和功能是脂肪酶固定化的关键。金属有机框架(metal-organic frameworks,MOFs)因孔径可调、易修饰、生物相容性好等优势成为固定脂肪酶的优良载体。该文首先介绍了MOFs的种类及其不同的合成方式,并论述其优缺点,再详细阐明脂肪酶在MOFs上的固定化方法,最后对MOFs-脂肪酶作为催化剂在酯交换反应中的应用进行讨论与总结归纳,为固定化酶及油脂转化提供理论和技术支撑。Enzymatic conversion of oils to fatty acid esters can simultaneously meet the needs of food safety and'double carbon'strategy.Unstable properties,difficult to recover and the high cost limit its large-scale industrial application of free lipase.The use of porous materials as carriers to immobilize lipases could overcome those problems,reasonable design of the structure and function of the carrier is the key to immobilize lipase.Metal-organic frameworks(MOFs)is an excellent carrier for lipase immobilization due to its advantages of adjustable pore size,easy modification and good biocompatibility.This article introduced the types of MOFs and their synthetic methods,and discussed their advantages and disadvantages.Then the immobilization methods for lipase loading in MOFs were detailed elucidated.And the application of MOFs-lipase as a catalyst in the transesterification were finally summarized.This study aims to provide theoretical and technical support for lipase immobilization and oil conversion.

关 键 词：脂肪酶 金属有机框架 固定化 酯交换 酶促反应 

分 类 号：Q814.2[生物学—生物工程]