保健食品中维生素K2定量分析方法的建立  

Establishment of a quantitative analysis method for vitamin K2 in health food

作　　者：王俊星 赵凯霞 陈笑南 吴茵茵 王新艳 WANG Junxing;ZHAO Kaixia;CHEN Xiaonan;WU Yinyin;WANG Xinyan(Chenguang Biotechnology Group Handan Co.,Ltd.,Handan 056100;Chenguang Bio-tech Group Co.,Ltd.,Quzhou 057250;Key Laboratory of Plant Resources Comprehensive Utilization in Hebei Province,Handan 056000)

机构地区：[1]晨光生物科技集团邯郸有限公司,邯郸056100 [2]晨光生物科技集团股份有限公司,曲周057250 [3]河北省植物资源综合利用重点实验室,邯郸056000

出　　处：《中国食品添加剂》2024年第9期223-228,共6页China Food Additives

摘　　要：本研究建立了测定含有维生素K2成分的保健食品中维生素K2含量的高效液相分析方法,采用Kromasil-100-5-C18色谱柱(4.6 mm×250 mm,5μm)进行分离,以甲醇-无水乙醇为流动相进行梯度洗脱;检测波长为270 nm;柱温为30℃;流速为1 mL/min;进样体积为20μL;外标法定量。结果显示该方法专属性强,阴性样品对目标峰无干扰;样品浓度在2~100μg/mL范围内线性关系良好,相关系数R^(2)=0.9999;检出限与定量限分别为0.078、0.625μg/mL;准确度和精密度(重复性、中间精密度)相对标准偏差符合要求。本文建立的检测方法操作简单,准确度、重现性均良好,适合用于保健食品中维生素K2含量的测定。An efficient liquid chromatographic analysis method was developed for the determination of vitamin K2 content in health food.Separation was achieved using a Kromasil-100-5-C18 column(4.6 mm×250 mm,5μm)with methanol-absolute ethanol as the gradient elution mobile phase.The detection wavelength was set at 270 nm,the column temperature was 30℃,the flow rate was 1 mL/min,and the injection volume was 20μL.Quantification was performed using an external standard method.The results demonstrated that the method showed high specificity,with no interference from the negative sample on the target peak.The linear relationship was good within the sample concentration range of 2~100μg/mL,with a correlation coefficient R^(2)=0.9999.The detection limit and quantification limit were 0.078 and 0.625μg/mL,respectively.The relative standard deviations of sample recovery rate and precision(repeatability,intermediate precision)met the requirements.The detection method established in this study is simple to operate,with good accuracy and reproducibility,the method is suitable for the determination of vitamin K2 content in health foods.

关 键 词：维生素K2 高效液相色谱法 含量检测 保健食品 方法学验证 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q568[轻工技术与工程—食品科学与工程]