Prognostic value of PEA3 subfamily gene expression in cholangiocarcinoma  

作　　者：Li Wang Zhe Zhang Hai-Zhang Ma 

机构地区：[1]Department of Emergency,The Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310000,Zhejiang Province,China [2]Department of Emergency Medicine,The First People’s Hospital of Linping District Hangzhou,Hangzhou 311100,Zhejiang Province,China [3]Department of General Surgery,Qilu Hospital of Shandong University,Jinan 250000,Shandong Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第9期4014-4027,共14页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Science and Technology Development Plan Project of Hangzhou,No.20201203B56.

摘　　要：BACKGROUND Cholangiocarcinoma(CCA)is a lethal malignancy with limited treatment options and poor prognosis.The PEA3 subfamily of E26 transformation specific genes:ETV1,ETV4,and ETV5 are known to play significant roles in various cancers by influencing cell proliferation,invasion,and metastasis.AIM To analyze PEA3 subfamily gene expression levels in CCA and their correlation with clinical parameters to determine their prognostic value for CCA.METHODS The expression levels of PEA3 subfamily genes in pan-cancer and CCA data in the cancer genome atlas and genotype-tissue expression project databases were analyzed with R language software.Survival curve and receiver operating characteristic analyses were performed using the SurvMiner,Survival,and Procr language packages.The gene expression profiling interactive analysis 2.0 database was used to analyze the expression levels of PEA3 subfamily genes in different subtypes and stages of CCA.Web Gestalt was used to perform the gene ontology/Kyoto encyclopedia of genes and genomes(GO/KEGG)analysis,and STRING database analysis was used to determine the genes and proteins related to PEA3 subfamily genes.RESULTS ETV1,ETV4,and ETV5 expression levels were significantly increased in CCA.There were significant differences in ETV1,ETV4,and ETV5 expression levels among the different subtypes of CCA,and predictive analysis revealed that only high ETV1 and ETV4 expression levels were significantly associated with shorter overall survival in patients with CCA.GO/KEGG analysis revealed that PEA3 subfamily genes were closely related to transcriptional misregulation in cancer.In vitro and in vivo experiments revealed that PEA3 silencing inhibited the invasion and metastasis of CCA cells.CONCLUSION The expression level of ETV4 may be a predictive biomarker of survival in patients with CCA.

关 键 词：PEA3 subfamily CHOLANGIOCARCINOMA The cancer genome atlas ETV4 The prognosis Prognostic biomarker 

分 类 号：R735.2[医药卫生—肿瘤]