Sulforaphane ameliorates non-alcoholic steatohepatitis by KLF4-mediated macrophage M2 polarization  

作　　者：Xianghui Huang Jia Xu Ye Xu Bingxin Huangfu Feng Zhang Yanzhou Hu Ruxin Gao Xinxin Ren Boyang Zhang Kunlun Huang Xiaoyun He 

机构地区：[1]Key Laboratory of Precision Nutrition and Food Quality,Key Laboratory of Functional Dairy,Ministry of Education,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China [2]Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),the Ministry of Agriculture and Rural Affairs of the P.R.China,Beijing 100083,China

出　　处：《Food Science and Human Wellness》2024年第5期2727-2740,共14页食品科学与人类健康（英文）

基　　金：supported by the Science and Technology project of Henan Province(202102310142);the National Natural Science Foundation of China(32001806)。

摘　　要：Non-alcoholic fatty liver disease (NAFLD) has become a global issue and a severe threat to public health.However, to date, no approved therapeutic drugs have been developed. Dietary interventions with naturalproducts have shown promise in preventing and treating NAFLD. Sulforaphane (SFN) is a phytocompoundwith antioxidant and anti-inflammatory properties, and previous research has demonstrated that SFN canameliorate hepatic lipid accumulation and inflammation. However, the molecular mechanisms underlying thesebeneficial effects remain unclear. In this study, we confirmed the protective effects of SFN on excessive lipidaccumulation and inflammatory injury in a high-fat, high-fructose diet-induced non-alcoholic steatohepatitis(NASH) mouse model. We found that SFN attenuates the inflammatory injury in a macrophage cell line andthe liver of NASH mice, owing to the promotion of M1-type macrophage polarization toward the M2-type andthe regulation of inflammatory mediators. Further analysis demonstrated that this SFN-induced macrophageM2-type polarization occurs in a Krüppel-like factor 4 (KLF4)-dependent manner. In summary, we uncovereda new mechanism of action underlying SFN activity and provide evidence that dietary intervention with SFNmight be protective against NASH.

关 键 词：Non-alcoholic steatohepatitis(NASH) Krüppel-like factor 4 Nuclear translocation CHEMOKINE Lipid metabolism 

分 类 号：TS201.4[轻工技术与工程—食品科学]