Oligomeric procyanidins combined with Parabacteroides distasonis ameliorate high-fat diet-induced atherosclerosis by regulating lipid metabolism,inflammation reaction and bile acid metabolism in ApoE^(-/-)mice  

作　　者：Mingjuan Xu Cheng Lü Yiqing Hu Mo Zhang Jinxin Shen Chunyi Liu Qun Lu Rui Liu 

机构地区：[1]College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]Wuhan Engineering Research Center of Bee Products on Quality and Safety Control,Wuhan 430070,China [3]Institute for Environment and Safety,Wuhan Academy of Agricultural Sciences,Wuhan 430070,China [4]Key Laboratory of Environment Correlative Dietology(Huazhong Agricultural University),Ministry of Education,Wuhan 430070,China [5]Key Laboratory of Urban Agriculture in Central China,Ministry of Agriculture and Rural Affairs,Wuhan 430070,China

出　　处：《Food Science and Human Wellness》2024年第5期2847-2856,共10页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(32272331)。

摘　　要：Atherosclerosis(AS)is the main pathological basis of cardiovascular diseases.Hence,the prevention and treatment strategies of AS have attracted great research attention.As a potential probiotic,Pararabacteroides distasonis has a positive regulatory effect on lipid metabolism and bile acids(BAs)profile.Oligomeric procyanidins have been confirmed to be conducive to the prevention and treatment of AS,whose antiatherosclerotic effect may be associated with the promotion of gut probiotics.However,it remains unclear whether and how oligomeric procyanidins and P.distasonis combined(PPC)treatment can effectively alleviate high-fat diet(HFD)-induced AS.In this study,PPC treatment was found to significantly decrease atherosclerotic lesion,as well as alleviate the lipid metabolism disorder,inflammation and oxidative stress injury in ApoE^(-/-)mice.Surprisingly,targeted metabolomics demonstrated that PPC intervention altered the BA profile in mice by regulating the ratio of secondary BAs to primary BAs,and increased fecal BAs excretion.Further,quantitative polymerase chain reaction(qPCR)analysis showed that PPC intervention facilitated reverse cholesterol transport by upregulating Srb1 expression;In addition,PPC intervention promoted BA synthesis from cholesterol in liver by upregulating Cyp7a1 expression via suppression of the farnesoid X receptor(FXR)pathway,thus exhibiting a significant serum cholesterol-lowering effect.In summary,PPC attenuated HFD-induced AS in ApoE^(-/-)mice,which provides new insights into the design of novel and efficient anti-atherosclerotic strategies to prevent AS based on probiotics and prebiotics.

关 键 词：ATHEROSCLEROSIS Pararabacteroides distasonis Oligomeric procyanidins Reverse cholesterol transport Bile acid metabolism 

分 类 号：TS201.4[轻工技术与工程—食品科学]