γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation  

作　　者：Yao Wang Dongwei Wang Kai Wang Min Zhao Cizhou Li Yu Wang Xuwei Liu Lei Zhao Zhuoyan Hu 

机构地区：[1]Guangdong Provincial Key Laboratory of Food Quality and Safety,College of Food Science,South China Agricultural University,Guangzhou 510642,China [2]Guangzhou Wanglaoji Lychee Industry Development Co.,Ltd.,Guangzhou 510623,China

出　　处：《Food Science and Human Wellness》2024年第5期3043-3053,共11页食品科学与人类健康（英文）

基　　金：supported by China Agriculture Research System of MOF and MARA(CARS-32);the Guangzhou Wanglaoji Lychee Industry Research Project(5100-H220577);the Science and Technology Planning Project of Guangzhou City of China(202103000054);the National Natural Science Foundation of China(32202022);the Dongguan Key R&D Programme(2022120030008).

摘　　要：Previous research reported litchi thaumatin-like protein(LcTLP)could lead to inflammation,which is a factor causing the adverse reactions after excessive intake of litchi.As a main amino acid in litchi pulp,γ-aminobutyric acid(GABA)was found with anti-inflammatory effect.Therefore,this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice models.In vitro study showed GABA could effectively regulate the level of inflammatory cytokines(interleukin(IL)-1β,IL-6,IL-10,and prostaglandin E2)and Ca2+in cells,and inhibit the phosphorylation of p65,IκB,p38,c-Jun N-terminal kinase(JNK)and extracellular signal-regulated kinase(ERK).These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling pathways.In vivo experiment was performed to verify the anti-inflammatory effect of GABA,and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice,as it down-regulated the pro-inflammatory cytokines,malondialdehyde,aspartate transferase,and alanine transaminase.The relative expression of phosphorylated p38,JNK and ERK in mice liver with GABA treatment were reduced to 65%,39%and 80%of the control group,respectively.Furthermore,GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut,which reveals GABA could effectively reduce the translocation of gut microbiota.

关 键 词：ANTI-INFLAMMATION Gut microbiota Litchi thaumatin-like protein Mitogen-activated protein kinase pathway γ-Aminobutyric acid 

分 类 号：TS201.4[轻工技术与工程—食品科学]