一株高产水溶性β-1,3-葡聚糖的棕鞭藻高密度异养培养  

High-cell-density heterotrophic cultivation of Ochromonas malhamensis with high water-solubleβ-1,3-glucan

作　　者：王谝 周广航 杨伊默 加晶 WANG Pian;ZHOU Guanghang;YANG Yimo;JIA Jing(Beijing Key Laboratory of Microalgae Bioenergy and Bioresource,Beijing 100142,China;SDIC BiotechnologyInvestment Co.,Ltd.,State Development and Investment Corporation,Beijing 100034,China)

机构地区：[1]微藻生物能源与资源北京市重点实验室,北京100142 [2]国投生物科技投资有限公司国投微藻生物科技中心,北京100034

出　　处：《发酵科技通讯》2024年第3期131-137,143,共8页Bulletin of Fermentation Science and Technology

基　　金：国家开发投资集团有限公司科技专项微藻生物技术的产业化应用开发项目(00003)。

摘　　要：棕鞭藻作为一种淡水藻类,能够用于生产水溶性β-1,3-葡聚糖。为了提高棕鞭藻的生物量,增加其水溶性β-1,3-葡聚糖的产量,笔者在7.5 L发酵罐中对棕鞭藻的异养培养基和异养培养条件进行了优化,同时也在125 L发酵罐中进行工艺放大验证。实验结果表明:采用优化后的培养基和培养条件,棕鞭藻在7.5 L发酵罐中培养,生物量达到61.25 g/L,与优化前相比提高了2.4倍;在125 L发酵罐中放大培养,棕鞭藻的生物量与7.5 L发酵罐中的培养结果相比无显著差异,水溶性β-1,3-葡聚糖的平均生产速率达到0.36 g/(L·h),较外籍文献报道的产率最高值0.13 g/(L·h)提高了1.8倍。该研究为棕鞭藻异养培养生产生物活性物质β-1,3-葡聚糖提供了依据,也为棕鞭藻在食品、医药和饲料等领域的应用创造了条件。As a freshwater alga,Ochromonas malhamensis can be used to produce water-solubleβ-1,3-glucan.In order to increase the biomass and yield of water-solubleβ-1,3-glucan of Ochromonas malhamensis,the heterotrophic medium and conditions of Ochromonas malhamensis were optimized in 7.5 L fermenter,and the process was scaled up in 125 L fermenter.The experimental results showed that with optimized medium and culture conditions,the biomass of Ochromonas malhamensis cultured in 7.5 L fermenter reached 61.25 g/L,which was 2.4 times higher than that before optimization.In 125 L fermenter,the biomass of Ochromonas malhamensis was not significantly different from that of 7.5 L fermenter.The average production rate of water-solubleβ-1,3-glucan reached 0.36 g/(L·h),which was 1.8 times higher than that reported in the literature.This study provided a basis for the production ofβ-1,3-glucan by high-cell-density heterotrophic cultivation of Ochromonas malhamensis,and laid a foundation for its application in food,medicine and feed.

关 键 词：棕鞭藻 水溶性β-1 3-葡聚糖 发酵罐 异养培养 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] S968.4[农业科学—水产养殖]