Utilizing engineered extracellular vesicles as delivery vectors in the management of ischemic stroke:a special outlook on mitochondrial delivery  

作　　者：Jiali Chen Yiyang Li Xingping Quan Jinfen Chen Yan Han Li Yang Manfei Zhou Greta Seng Peng Mok Ruibing Wang Yonghua Zhao 

机构地区：[1]Institute of Chinese Medical Sciences,State Key Laboratory of Quality Research in Chinese Medicine,University of Macao,Taipa,Macao Special Administrative Region,China [2]Department of Pharmacy,Hunan Provincial People’s Hospital,the First Affiliated Hospital of Hunan Normal University,Changsha,Hunan Province,China [3]Department of Electrical and Computer Engineering,University of Macao,Taipa,Macao Special Administrative Region,China [4]Department of Pharmaceutical Sciences,Faculty of Health Sciences,University of Macao,Taipa,Macao Special Administrative Region,China

出　　处：《Neural Regeneration Research》2025年第8期2181-2198,共18页中国神经再生研究（英文版）

基　　金：supported by the grants from University of Macao,China,Nos.MYRG2022-00221-ICMS(to YZ)and MYRG-CRG2022-00011-ICMS(to RW);the Natural Science Foundation of Guangdong Province,No.2023A1515010034(to YZ)。

摘　　要：Ischemic stroke is a secondary cause of mortality worldwide,imposing considerable medical and economic burdens on society.Extracellular vesicles,serving as natural nanocarriers for drug delivery,exhibit excellent biocompatibility in vivo and have significant advantages in the management of ischemic stroke.However,the uncertain distribution and rapid clearance of extracellular vesicles impede their delivery efficiency.By utilizing membrane decoration or by encapsulating therapeutic cargo within extracellular vesicles,their delivery efficacy may be greatly improved.Furthermore,previous studies have indicated that microvesicles,a subset of large-sized extracellular vesicles,can transport mitochondria to neighboring cells,thereby aiding in the restoration of mitochondrial function post-ischemic stroke.Small extracellular vesicles have also demonstrated the capability to transfer mitochondrial components,such as proteins or deoxyribonucleic acid,or their sub-components,for extracellular vesicle-based ischemic stroke therapy.In this review,we undertake a comparative analysis of the isolation techniques employed for extracellular vesicles and present an overview of the current dominant extracellular vesicle modification methodologies.Given the complex facets of treating ischemic stroke,we also delineate various extracellular vesicle modification approaches which are suited to different facets of the treatment process.Moreover,given the burgeoning interest in mitochondrial delivery,we delved into the feasibility and existing research findings on the transportation of mitochondrial fractions or intact mitochondria through small extracellular vesicles and microvesicles to offer a fresh perspective on ischemic stroke therapy.

关 键 词：delivery engineering extracellular vesicles identification ischemic stroke isolation MITOCHONDRIA targeting strategy therapeutic effects 

分 类 号：R743.3[医药卫生—神经病学与精神病学]