水产品肠道中富硒乳酸菌的筛选及其体外生物活性  

Screening of Selenium⁃Rich Lactic Acid Bacteria from Intestinal Tract of Aquatic Products and Evaluation of Its Bioactivities in vitro

作　　者：张艳芳 吕欣然 武文玉 白凤翎 崔方超 檀茜倩 励建荣 李英美 ZHANG Yanfang;LÜXinran;WU Wenyu;BAI Fengling;CUI Fangchao;TAN Xiqian;LI Jianrong;LI Yingmei(National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,School of Food Science and Engineering,Bohai University,Jinzhou 121013,Liaoning,China;Dalian Food(Jinzhou)Co.,Ltd.,Jinzhou 121019,Liaoning,China;Collaborative Innovation Center for Key Technologies of Marine Food Intensive Processing,Dalian Polytechnic University,Dalian 116034,Liaoning,China)

机构地区：[1]渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]达莲食品(锦州)有限公司,辽宁锦州121019 [3]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

出　　处：《食品研究与开发》2024年第18期106-113,共8页Food Research and Development

基　　金：海参精深加工关键技术研发及产业化(辽自然资发(2021)84号);辽宁省“兴辽英才计划”青年拔尖人才项目(XLYC1807133)。

摘　　要：从来源于水产动物肠道的8株乳酸菌中筛选富硒活性较好的菌株,并对其抑菌能力、耐胆盐、抗氧化能力及Caco⁃2细胞的黏附性进行分析。结果表明:来源于花鲢鱼肠道的菌株ZHL⁃2的富硒率高达85.46%,经分子生物学鉴定为植物乳杆菌(Lactiplantibacillus plantarum)。与未富硒菌株ZHL⁃2相比,富硒后菌株ZHL⁃2完整细胞和破碎细胞提取物清除DPPH自由基和超氧阴离子的能力分别提高3.67%、3.67%、11.67%、11.00%。富硒后菌株ZHL⁃2胆盐耐受能力以及对Caco⁃2细胞的黏附数分别增加6.54%和7.34 CFU/个,对大肠杆菌及金黄色葡萄球菌的抑菌圈直径分别增加1.14 mm和2.89 mm。Strains with better selenium⁃rich activity were selected from eight lactic acid bacteria(LAB)origi⁃nating from the intestinal tract of aquatic animals,and their antibacterial ability,bile salt resistance,antioxi⁃dant ability,and adhesion to Caco⁃2 cells were analyzed.The results showed that strain ZHL⁃2 from the intesti⁃nal tract of silver carp had a selenium⁃rich rate of 85.46%and was identified as Lactiplantibacillus plantarum by molecular biology.Compared with that of strain ZHL⁃2 without rich selenium,the scavenging ability of DPPH radical and superoxide anion of intact cells and cell⁃free extracts from selenium⁃rich strain ZHL⁃2 in⁃creased by 3.67%,3.67%,11.67%,and 11.00%,respectively.The bile salt resistance and adhesion to Caco⁃2 cells of selenium⁃rich strain ZHL⁃2 increased by 6.54%and 7.34 CFU/cell,respectively,and the inhibition zone diameter against Escherichia coli and Staphylococcus aureus increased by 1.14 mm and 2.89 mm.

关 键 词：水产品肠道 乳酸菌 筛选 富硒 生物活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程] TS201.3[轻工技术与工程—食品科学与工程]