高温产2,3-丁二醇甲基芽孢杆菌的筛选及其发酵优化  

Screening of thermophilic Bacillus methylotrophicus for efficient 2,3-butanediol production and its fermentation optimization

作　　者：林燕环 陈飞雪 魏宸鑫 李润志 张燎原[2] 林辉[2,3] LIN Yanhuan;CHEN Feixue;WEI Chenxin;LI Runzhi;ZHANG Liaoyuan;LIN Hui(College of Food Engineering,Zhangzhou Institute of Technology,Zhangzhou 363000,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Edible Fungi,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)

机构地区：[1]漳州职业技术学院食品工程学院,福建漳州363000 [2]福建农林大学生命科学学院,福建福州350002 [3]福建省农业科学院食用菌研究所,福建福州350013

出　　处：《食品与发酵工业》2024年第18期98-106,共9页Food and Fermentation Industries

基　　金：国家自然科学基金项目(81673542);福建省自然科学基金项目(2023J05067)。

摘　　要：2,3-丁二醇是一种典型的大宗化学品,广泛应用于化工、食品、医药、燃料等领域。生物法发酵制备2,3-丁二醇是当下的主要方法,然而,大多数报道的菌株为条件致病菌且发酵条件严格,限制了生物法制备2,3-丁二醇的发展。该研究以温泉底土壤为试验材料,经过分离、筛选与鉴定获得了1株高温条件下产2,3-丁二醇的甲基芽孢杆菌。通过摇瓶实验优化发酵工艺,确定最适发酵条件与最优培养基组成,利用优化后的发酵工艺甲基芽孢杆菌发酵24 h产物产量达到32.57 g/L。进一步进行上罐试验,基于不同发酵罐转速条件下甲基芽孢杆菌的产量积累情况,理性设计两阶段发酵策略,并以最优策略分批补料发酵制备2,3-丁二醇,2,3-丁二醇的产量在甲基芽孢杆菌发酵45 h时达到73.19 g/L,转化率达到90.25%。2,3-Butanediol,an important bulk chemical,is widely used in the field of chemical industry,food,pharmaceutical and fuel.Biological 2,3-butanediol production via microbial fermentation is the main method at present.However,several reported strains with high yields are opportunistic pathogen,and strict fermentation conditions are required,which limited the progress of biological 2,3-butanediol production.In this study,a newly isolated Bacillus methylotrophicus strain that thermophilically produces 2,3-butanediol was screened and identified from the soil of hot spring.The optimized fermentation conditions and medium components resulted in 2,3-butanediol production with 32.57 g/L at 24 h.Furthermore,the fermentation experiment in a 5 L bioreactor was performed,and the effects of different agitation speeds on 2,3-butanediol production were investigated.Ultimately,a two-stage fermentation strategy combined with fed-batch was developed,and 73.19 g/L 2,3-butanediol with the yield of 90.25%was achieved in a 5 L bioreactor at 45 h.

关 键 词：2 3-丁二醇 甲基芽孢杆菌 高温发酵 发酵优化 分批补料发酵 

分 类 号：TQ923[轻工技术与工程—发酵工程]