复方保健酒中8种有效成分检测及其稳定性分析  

Study on the detection and stability of 8 effective components in compound health wine

作　　者：胡杨 罗欣 刘洋 解倩倩 黄智安 沈蕊 HU Yang;LUO Xin;LIU Yang;XIE Qianqian;HUANG Zhi'an;SHENRui(Jing Brand Co,Ltd,Daye 435100,China;Hubei Key Laboratory of Quality and Safety of Traditional Chinese Medicine Health Food,Daye 435100.China)

机构地区：[1]劲牌有限公司,湖北大冶435100 [2]中药保健食品质量与安全湖北省重点实验室,湖北大冶435100

出　　处：《中国酿造》2024年第9期228-233,共6页China Brewing

基　　金：湖北重点研发计划(2020BBA050)。

摘　　要：该研究采用高效液相色谱(HPLC)法建立测定复方保健酒中8种有效成分(5-羟甲基糠醛、腺苷、虫草素、N6-(2-羟乙基)腺苷、苦番红花素、芦丁、西红花苷Ⅰ、西红花苷Ⅱ)的方法,并对酒样中8种有效成分的稳定性进行分析。结果表明,最佳色谱条件为:采用Agilent SB-Aq C18色谱柱(4.6 mm×250 mm, 5μm),以乙腈-0.1%乙酸水溶液为流动相,流速1.0 mL/min,检测波长260 nm/440 nm,柱温30℃,进样量10μL。在该分析条件下,8种有效成分在各自质量浓度范围内线性关系良好(R^(2)≥0.999 9),检出限(LOD)为15.5~146.1μg/L、定量限(LOQ)为45.5~429.7μg/L,精密度、稳定性、重复性试验结果的相对标准偏差(RSD)均<2.0%,平均加标回收率为96.15%~99.23%。样品稳定性试验结果表明,在不同的贮存条件下贮存90 d,腺苷、虫草素、N6-(2-羟乙基)腺苷、苦番红花素稳定性较好;5-羟甲基糠醛对温度较敏感;芦丁、西红花苷Ⅰ、西红花苷Ⅱ对光和热均极其敏感。Amethod for the determination of 8 effective components(5-hydroxymethylfurfural,adenosine,cordycepin,N6-(2-hydroxyethyl)adenosine,picrocrocin,rutin,crocin I and crocin II in compound health wine was established by HPLC,and the stability of 8 effective components was analyzed.The results showed that the optimal chromatographic conditions were as follows:Agilent SB-Aq C_(18) column(4.6 mm×250 mm,5μm),acetonitrile-0.1%acetic acid aqueous as mobile phase,flowrate 1.0 ml/min,detection wavelength 260 nm/440 nm,column temperature 30℃,and injection volume 10μl.Under the analytical conditions,the 8 effective ingredients showed good linear relationships in their respective mass concentration ranges(R^(2)≥0.9999).The limits of detection(LOD)were 15.5-146.1μg/L,and the limits of quantitation(LOQ)were 45.5-429.7μg/L.The relative standard deviations(RSD)of precision,stability and repeatability tests were all lower than 2.0%.The average spiked recovery rates were 96.15%-99.23%.The stability tests results of samples showed that the adenosine,cordycepin,N6-(2-hydroxyethyl)adenosine and picrocrocin were stable under different storage conditions for 90 d,the 5-hydroxymethylfurfural was sensitive to temperature,and the rutin,crocin I,and crocin II were extremely sensitive to light and heat.

关 键 词：高效液相色谱法 复方保健酒 有效成分 稳定性 

分 类 号：TS262.7[轻工技术与工程—发酵工程]