HOG1基因缺失对酵母细胞锌耐受的影响  

Effects of HOG1 Gene Deletion on Zinc Tolerance of Yeast Cells

作　　者：葛文君 吴丽华[1] 陈燕飞[1] 赵文婧[1] GE Wenjun;WU Lihua;CHEN Yanfei;ZHAO Wenjing(College of Biological Science and Technology,Taiyuan Normal University,Jinzhong Shanxi 030619,China)

机构地区：[1]太原师范学院生物科学与技术学院,山西晋中030619

出　　处：《太原师范学院学报（自然科学版）》2024年第3期65-70,共6页Journal of Taiyuan Normal University:Natural Science Edition

基　　金：国家自然科学基金(21307087);山西省应用基础研究计划青年科技研究基金(201601D021104);山西省大学生创新创业项目(20230917)。

摘　　要：以酿酒酵母(ΔHOG1突变株和野生株BY4741)为材料,检测了酵母细胞在硫酸锌胁迫下生长、凋亡、ROS水平和线粒体膜电位的变化.结果显示,高浓度硫酸锌可抑制酵母细胞生长,引起胞内ROS水平升高和线粒体膜电位下降,进而诱导细胞发生凋亡.在3 mmol/L的硫酸锌处理组中,ΔHOG1突变株的锌耐受性和线粒体膜电位显著低于野生株BY4741,而胞内ROS水平和细胞凋亡率显著高于野生株BY4741.结果表明,HOG1 MAPK可通过影响胞内ROS水平调控酵母细胞对硫酸锌的耐受性.In this experiment,yeast wild-type(BY4741)and HOG1 mutant(△HOG1)strains were used as materials to detect the changes of growth,apoptosis,intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential of yeast cells under zinc sulfate stress.The results showed that high concentration of zinc inhibits cell growth,causes the level of intracellular ROS increased,and induced apoptosis.At the concentration of 3 mmol/L zinc sulfate treatment group,zinc tolerance and mitochondrial membrane potential were lower,meanwhile,the intracellular ROS level and apoptosis rate were higher significantly in the yeast strain lacking HOG1(△HOG1)compared to the strain BY4741.These results indicated that HOG1 may regulate the zinc sulfate tolerance of yeast cells by controlling the generation of intracellular ROS.

关 键 词：酵母 硫酸锌 凋亡 活性氧 HOG1 

分 类 号：Q935[生物学—微生物学] X171.5[环境科学与工程—环境科学]