大麦籽粒β-葡聚糖含量的全基因组关联分析及候选基因预测  

Genome-wide association analysis and candidate genes prediction ofβ-glucan content in barley grains

作　　者：鲁宗辉 司二静[1,2] 叶霈颖 汪军成[1,2] 姚立蓉 马小乐[1,2] 李葆春[1,3] 王化俊[1,2] 尚勋武[2] 孟亚雄[1,2] LU Zong-Hui;SI Er-Jing;YE Pei-Yin;WANG Jun-Cheng;YAO Li-Rong;MA Xiao-Le;LIBao-Chun;WANG Hua-Jun;SHANG Xun-Wu;MENG Ya-Xiong(State Key Laboratory of Arid Land Crop Science,Gansu Provincial Key Laboratory of Crop Improvement&Germplasm Enhancement,Lanzhou 730070,Gansu,China;College of Agriculture,Gansu Agricultural University,Lanzhou 730070,Gansu,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《作物学报》2024年第10期2483-2492,共10页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(大麦青稞,CARS-05-03B-03);国家自然科学基金项目(31960426);甘肃省省级大学生创新创业训练计划(S202010733001);甘肃省青年科技基金计划(20JR5RA010);甘肃省教育厅创新基金(2021A-055);甘肃省教育厅产业支撑计划(2021CYZC-12)资助。

摘　　要：利用高通量SNP芯片对238份不同来源的大麦种质资源进行基因型分析,并测定2年的籽粒β-葡聚糖含量,通过基于PCA模型的一般线性模型(general linear model,GLM)进行全基因组关联分析(genome-wide association study,GWAS)。结果表明,238份大麦材料的β-葡聚糖含量分布在1.23%~6.55%和1.79%~6.64%之间且均呈正态分布。GWAS分析共检测到19个显著的SNP标记,分布在1H、2H、3H、4H和5H染色体上,可解释表型变异的7.39%~10.29%。在显著关联的SNP位点上下游各300 kb范围内进行候选基因挖掘,共寻找到37个基因,基于前人研究和BLAST基因注释共筛选到4个最有可能与β-葡聚糖合成相关的候选基因,在最显著SNP位点B1_1033963上游89 kb处找到候选基因HORVU.MOREX.r3.1HG0000140,该基因可能是与β-葡聚糖合成过程紧密相关的基因。本研究可为大麦β-葡聚糖含量遗传改良提供理论指导与优异基因资源。A total of 238 barley germplasm resources from various sources were genotyped using a high-throughput SNP chip.Theβ-glucan content of barley grain was determined for two consecutive years.Genome-wide association analysis was conducted using a general linear model based on the PCA model.The results showed that theβ-glucan content of the 238 barley materials ranged from 1.23%to 6.55%and 1.79%to 6.64%for the respective years,exhibiting a normal distribution.In the GWAS analysis,a total of 19 significant SNP markers were detected,located on chromosomes 1H,2H,3H,4H,and 5H.These markers accounted for 7.39%to 10.29%of the observed phenotypic variation.Candidate genes were identified within a 300 kb range upstream and downstream of the SNP sites showing significant associations,and a total of 37 genes were identified.Through a combination of previous studies and BLAST gene annotation,four candidate genes most likely involved inβ-glucan synthesis were identified.Notably,within 89 kb upstream of the most significant SNP locus B1_1033963,the candidate gene HORVU.MOREX.R3.1 HG0000140 was detected,which may play a crucial role in the process of beta-glucan synthesis.This study provides theoretical guidance and valuable gene resources for the genetic improvement ofβ-glucan content in barley.

关 键 词：大麦 Β-葡聚糖 全基因组关联分析 SNP标记 

分 类 号：S512.3[农业科学—作物学]