AFM1劈裂适配体的识别能力研究  

Investigation on the Recognition of Split Aflatoxin M1 Aptamer

作　　者：郑业坤 杜聪聪 李泓霖 郭婷[1,2,3] 张宇昊 马良[1,2,3] ZHENG Yekun;DU Congcong;LI Honglin;GUO Ting;ZHANG Yuhao;MA Liang(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing,Chongqing 400715,China;Key Laboratory of Condiment Supervision Technology for State Market Regulation,Chongqing 400715,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]川渝共建特色食品重庆市重点实验室,重庆400715 [3]国家市场监管重点实验室(调味品监管技术),重庆400715

出　　处：《食品工业科技》2024年第19期296-306,共11页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(32072137);国家自然科学基金联合基金项目(U22A20551);重庆市自然科学基金(cstc2021jcyj-msxmX0371)。

摘　　要：本研究以黄曲霉毒素M1为研究对象,采用劈裂适配体的方式,克服适配体构象不稳定造成的灵敏度不高的问题,利用电化学方法研究劈裂适配体的识别能力及特异性,并通过圆二色谱初步探究结合机制。结果表明,将适配体核苷酸链按照1:1进行劈裂得到的识别效果最佳,并且对黄曲霉毒素M1具有较高的特异性。劈裂适配体后单独两个片段与AFM1作用后圆二色谱图没有改变,正峰强度明显增大,负峰移动并且强度增大,证明适配体构象发生改变。基于劈裂适配体可以改善方法的灵敏度,通过对完整适配体进行劈裂,得到了构建传感器的更佳识别元件。本研究为提高适配体快速检测方法的灵敏度提供了一种新思路。In this work,AFM1 was chosen as the target,and the aptamer was split to solve the low sensitivity due to unstable structure of the aptamer.The recognition ability and specificity of the split aptamer were evaluated using electrochemical methods,and the binding mechanism was explored through the circular dichroism.The results showed that the split aptamer employing a 1:1 split mode exhibited the best performance in the recognition and specificity for AFM1.The circular dichroism spectrum remained unchanged.The positive peak intensity of the circular dichroism spectrum increased significantly and the negative peak moved and the intensity increased,which demonstrated that the conformation of the aptamer changed.The sensitivity was enhanced by employing split aptamers.Superior recognition elements for the construction of sensors was obtained by the fragmentation of intact aptamers.The investigation provides a new idea to enhance the sensitivity of the detection mehtods based on the apatamer.

关 键 词：黄曲霉毒素M1 劈裂适配体 识别能力 电化学 

分 类 号：O657.1[理学—分析化学] TS207.5[理学—化学]