硫酸软骨素蛋白多糖4预后价值的泛癌分析  

Pan-cancer Analysis of Prognostic Value of Chondroitin Sulfate Proteoglycan 4

作　　者：徐鸿学 谢俞宁 崔彤 张雪梅 XU Hong-xue;XIE Yu-ning;CUI Tong;ZHANG Xue-mei(School of Public Health,,North China University of Science and Technology,Tangshan 063210,Hebei,China;College of Life Sciences,North China University of Science and Technology,Tangshan 063210,Hebei,China)

机构地区：[1]华北理工大学公共卫生学院,河北唐山063210 [2]华北理工大学生命科学学院,河北唐山063210

出　　处：《医学信息》2024年第18期6-11,共6页Journal of Medical Information

基　　金：2021年度唐山市人力资源和社会保障局项目(编号:A202110007)。

摘　　要：目的分析CSPG4在不同肿瘤中的表达对预后的影响及其可能作用的生物学通路。方法使用TIMER和UALCAN分析CSPG4在泛癌中mRNA和蛋白质的表达情况,GEPIA分析癌组织和癌旁组织中CSPG4表达差异及其对肿瘤患者生存时间的影响。使用R语言clusterProfiler和org.Hs.eg.db程序包对CSPG4进行基因本体论(GO)功能富集分析,clusterProfiler和pathview程序包进行京都基因与基因组百科全书(KEGG)通路富集分析。用于GO和KEGG通路富集分析的CSPG4表达数据及相应的临床信息均来自于癌症基因组图谱(TCGA)数据库。结果在多形性胶质细胞瘤和头颈鳞状细胞癌的癌组织中,CSPG4在mRNA和蛋白水平均呈高表达。在乳腺癌、结肠癌、肺腺癌和子宫内膜癌中呈低表达。预后分析显示,CSPG4在肺腺癌中高表达,与肿瘤患者的不良预后显著相关(HR=1.4,P<0.05)。GO功能富集分析结果显示,CSPG4相关基因主要富集在肌动蛋白结合、细胞外基质结构成分和糖胺聚糖结合等。KEGG通路富集分析结果显示,CSPG4作用的信号通路主要集中于粘着斑、人乳头瘤病毒感染以及PI3K-Akt信号通路。结论CSPG4可能通过与机动蛋白结合,影响细胞外基质组分或通过PI3K/AKT信号通路参与肺腺癌的发生发展过程,影响肺腺癌的预后,CSPG4有可能作为肺腺癌的潜在生物标志物。Objective To analyze the effect of CSPG4 expression in different tumors on prognosis and its possible biological pathways.Methods TIMER and UALCAN were used to analyze the expression of CSPG4 mRNA and protein in pan-cancer tissues.GEPIA was used to analyze the difference of CSPG4 expression between cancer tissues and adjacent tissues and its effect on the survival time of cancer patients.Gene ontology(GO)functional enrichment analysis of CSPG4 was performed using the R language clusterProfiler and org.Hs.eg.db package.ClusterProfiler and pathview package were used for Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.The CSPG4 expression data and corresponding clinical information for GO and KEGG pathway enrichment analysis were from the Cancer Genome Atlas(TCGA)database.Results CSPG4 was highly expressed at both mRNA and protein levels in glioblastoma multiforme and head and neck squamous cell carcinoma,it was lowly expressed in breast cancer,colon cancer,lung adenocarcinoma and endometrial cancer.Prognostic analysis showed that CSPG4 was highly expressed in lung adenocarcinoma and was significantly associated with poor prognosis of tumor patients(HR=1.4,P<0.05).GO functional enrichment analysis showed that CSPG4-related genes were mainly enriched in actin binding,extracellular matrix structural components and glycosaminoglycan binding.The results of KEGG pathway enrichment analysis showed that the signaling pathways of CSPG4 were mainly focused on focal adhesion,human papillomavirus infection and PI3K-Akt signaling pathway.Conclusion CSPG4 may be involved in the occurrence and development of lung adenocarcinoma by binding to the motor protein,affecting the extracellular matrix components or through the PI3K/AKT signaling pathway,as well as affecting the prognosis of lung adenocarcinoma.Thus,CSPG4 may be used as a potential biomarker for lung adenocarcinoma.

关 键 词：硫酸软骨素蛋白多糖4 泛癌 肺腺癌 生存预后 基因突变 

分 类 号：R730.7[医药卫生—肿瘤]