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作 者:吴曜廷 刘丹然 李润杰 陈敬悦 杨军 李作森 徐晨元 WU Yao-ting;LIU Dan-ran;LI Run-jie(Yunnan Tobacco Leaf Company,Kunming,Yunnan 650217)
机构地区:[1]云南省烟草烟叶公司,云南昆明650217 [2]云南农业大学烟草学院,云南昆明650201
出 处:《安徽农业科学》2024年第18期166-170,共5页Journal of Anhui Agricultural Sciences
基 金:云南省烟草烟叶公司科技计划项目(2022530000216101)。
摘 要:为改善烟叶醇化品质,以低次红花大金元上部烟叶为试材,将BioApex微生物酶应用于烟叶醇化中,比较醇化烟叶的常规化学成分、挥发性香气成分和感官质量的变化。结果表明,添加微生物酶制剂会增加除氯以外的其他常规化学成分含量;同时降低了两糖差,使烟叶成分更加协调。各微生物酶制剂处理烟叶中还原糖、水溶性糖含量与CK相比均显著增加,但醇化时间延长会显著降低烟碱和水溶性糖含量。微生物酶制剂处理增加了烟叶中芳香族氨基酸降解产物、类胡萝卜素降解产物、美拉德反应产物含量。与CK相比,0.5%、1.0%和2.0%微生物酶制剂处理(90 d)明显改善了烟叶的感官质量。综上所述,微生物酶制剂能够促进烟叶内含物质的转化,提升红花大金元上部烟叶的品质。该研究可为烟叶微生物酶制剂发酵工艺在工业中的应用提供理论基础。In order to improve the aging quality of tobacco leaves,using the low-grade and upper tobacco leaves of Honghua Dajinyuan as the test material,the BioApex microbial enzyme was applied to tobacco leaves,and the conventional chemical components,volatile aroma composition and sensory quality of aged tobacco leaves were compared.The results showed that the content of conventional chemical components except chlorine were increased by microbial enzymes.Simultaneously,the microbial enzymes decreased the difference between the content of total sugars and reducing sugar,and coordinated chemical composition of aged leaves.With the increases of enzyme concentration,the content of reducing sugar,water-soluble sugar and potassium in tobacco leaves were also significantly increased.However,prolonged aging time will significantly reduce the content of nicotine and water-soluble sugar.Microbial enzyme treatment increased the degradation products of aromatic amino acids,carotenoids,and Maillard reactions in tobacco leaves.The sensory quality of tobacco leaves treated with 0.5%,1.0%and 2.0%(90 days)concentration of microbial enzyme were obviously improved compared with the control.In summary,microbial enzymes can promote the transformation of tobacco leaf contents and effectively improve the quality of the upper tobacco leaves.This study can provide theoretical basis for the industrial application of microbial enzyme preparation fermentation technology in tobacco leaves.
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