Double-layer probiotic encapsulation for enhanced bacteriotherapy against inflammatory bowel disease  

作　　者：Yixin Wang Jun Liu Sichen Yuan Yawen You Quanyin Hu 

机构地区：[1]Pharmaceutical Sciences Division,School of Pharmacy,University of Wisconsin-Madison,Madison,WI 53705,USA [2]Carbone Cancer Center,School of Medicine and Public Health,University of Wisconsin-Madison,Madison,WI 53705,USA [3]Wisconsin Center for NanoBioSystems,School of Pharmacy,University of Wisconsin-Madison,Madison,WI 53705,USA

出　　处：《Nano Research》2024年第10期9103-9110,共8页纳米研究（英文版）

基　　金：supported by the University of Wisconsin Carbone Cancer Center Research Collaborative;the Pancreas Cancer Task Force and the start-up package from the University of Wisconsin-Madison.

摘　　要：Inflammatory bowel disease(IBD)is inflammatory intestinal disorders associated with dysregulated gut microbiota.Bacteriotherapy that leverages bacteria as therapeutics has shown tremendous promise in resolving gut dysbiosis and reducing inflammatory mediators to treat IBD.Orally delivered probiotics,such as Escherichia coli Nissle 1917(EcN),can produce beneficial ingredients,competitively inhibit the proliferation of pathogens,and promote the restoration of gut microbiome homeostasis.However,environmental stresses(such as gastric acids)in the gastrointestinal(GI)tract pose an enormous challenge to the probiotics following oral administration,leading to decreases in viability and activity of probiotics.Meanwhile,the inferior mucoadhesive capability of probiotics results in low colonization efficacy,further compromising their therapeutic effect.Coating probiotics with functional biomaterials may protect them from elimination and prolong their retention in the GI tract.Here,we developed a facile double-layer electrostatic assembly technique to encapsulate EcN bacteria in protective layers of mucoadhesive chitosan(CS)and immunomodulatory hyaluronic acid(HA)to generate HA-CS-EcN.These biomaterials confer the coated EcN resistance to environmental assault and enhanced mucoadhesion in the GI tract.The probiotics equipped with the multifunctional shield can thus suppress inflammation and reshape the intestinal microenvironment to enhance therapeutic efficacy for the prevention and treatment of IBD.Collectively,this study presents a novel probiotic coating strategy to augment the outcome of bacteriotherapy to combat IBD.

关 键 词：PROBIOTICS biomaterial coating inflammatory bowel disease MUCOADHESION oral administration 

分 类 号：R574[医药卫生—消化系统]