葡萄干总黄酮和多酚成分的富集纯化及其对卷烟抽吸质量的影响  

Enrichment and Purification of Total Flavonoids and Polyphenols of Raisins and Their Effects on Cigarette Smoking Quality

作　　者：吴静[1] 谢传奇 王璐[1] 吴磊[1,2] 邓宇 WU Jing;XIE Chuanqi;WANG Lu;WU Lei;DENG Yu(Institute of Applied Chemistry,Jiangxi Academy of Sciences,Nanchang 330096,China;Technology Center of China Tobacco Jiangxi Industrial Co.,Ltd.,Nanchang 330096,China)

机构地区：[1]江西省科学院应用化学研究所,江西南昌330096 [2]江西中烟工业有限责任公司技术中心,江西南昌330096

出　　处：《生物化工》2024年第4期24-29,共6页Biological Chemical Engineering

基　　金：江西省科学院重大产业技术攻关专项(2020-YZD-1);江西省科学院杰出青年人才培育计划项目(2021YSBG50005)。

摘　　要：为解决植物粗提物在卷烟中的应用受限问题,充分发挥天然植物多元成分在卷烟加香加料的作用,利用纯水、50%乙醇溶液和90%乙醇溶液对葡萄干进行提取得到粗提物,利用D101大孔树脂通过不同浓度乙醇溶液洗脱对葡萄干粗提物进行纯化,得到葡萄干精制物,采用紫外分光光度法检测精制前后的总黄酮和多酚的含量变化,评价葡萄干粗提物、精制物在卷烟中的应用效果。结果表明:(1)不同提取溶剂获得的葡萄干粗提物总黄酮含量差异明显,50%乙醇粗提物总黄酮含量最高,为4.437 9 mg/g,粗提物中均不含多酚;(2)经大孔树脂富集后,30%乙醇洗脱流份中的总黄酮和多酚含量最高,纯水、50%乙醇和90%乙醇提取物的总黄酮含量分别为7.21%、16.04%和15.43%,多酚含量分别达到3.72%、5.50%、7.66%,实现了有效成分的富集;(3)葡萄干精制物对卷烟烟气的修饰作用明显优于粗提物,其中10%乙醇和30%乙醇洗脱精制物在柔和烟气、增加烟气量和改善抽吸品质方面效果更佳。In order to solve the problem of limited application of crude extract of natural flavor raw materials in cigarettes and give full play to the role of natural plant ingredients in cigarette flavoring and feeding,the raisins are extracted with pure water and different proportions of ethanol to obtain crude extract,and the raisins are enriched with D101 macroporous resin to obtain refined raisins,and the content changes of total flavonoids and total polyphenols before and after refining are detected by ultraviolet spectrophotometry,to evaluate the application effect of raisin extract in cigarettes.The results show that:(1) the content of total flavonoids and polyphenols in the crude extract of raisin with different extraction solvents is significantly different,and the content of total flavonoids in the crude extract of 50% ethanol is the highest,4.437 9 mg/g,none of the crude extracts containes polyphenols;(2) after enrichment by macroporous resin,the content of total flavonoids and polyphenols in 30% fluid is the highest,the contents of total flavonoids in water,50% ethanol and 90% ethanol extracts are 7.21%,16.04% and 15.43%,respectively,and the contents of polyphenols are 3.72%,5.50% and 7.66%,respectively,realizing the enrichment of effective components;(3) the 10% ethanol and 30% ethanol refined extracts has the advantages of improving cigarette smoke sensory quality,especially on smoothness.

关 键 词：葡萄干 大孔树脂 卷烟 总黄酮 多酚 

分 类 号：TS452.1[农业科学—烟草工业]