A role for Retinoblastoma 1 in hindbrain morphogenesis by regulating GBX family  

作　　者：Shuang Zhao Chen Wang Haiping Luo Feifei Li Qiang Wang Jin Xu Zhibin Huang Wei Liu Wenqing Zhang 

机构地区：[1]The Innovation Centre of Ministry of Education for Development and Diseases,School of Medicine,South China University of Technology,Guangzhou,Guangdong 510006,China [2]Greater Bay Biomedical Innocenter,Shenzhen Bay Laboratory,Shenzhen,Guangdong 518055,China

出　　处：《Journal of Genetics and Genomics》2024年第9期900-910,共11页遗传学报（英文版）

基　　金：supported by the National Key R&D Program of China(2018YFA0801000);the Guangdong Basic and Applied Basic Research Foundation(2023A1515010396);the Science and Technology。

摘　　要：The hindbrain,which develops from the anterior end of the neural tube expansion,can differentiate into the metencephalon and myelencephalon,with varying sizes and functions.The midbrain–hindbrain boundary(MHB)and hindbrain myelencephalon/ventral midline(HMVM)are known to be the source of the progenitors for the anterior hindbrain and myelencephalon,respectively.However,the molecular networks regulating hindbrain morphogenesis in these structures remain unclear.In this study,we show that retinoblastoma 1(rb1)is highly expressed at the MHB and HMVM in zebrafish.Knocking out rb1 in mice and zebrafish results in an enlarged hindbrain due to hindbrain neuronal hyperproliferation.Further study reveals that Rb1 controls the hindbrain morphogenesis by suppressing the expression of Gbx1/Gbx2,essential transcription factors for hindbrain development,through its binding to E2f3/Hdac1,respectively.Interestingly,we find that Gbx1 and Gbx2 are expressed in different types of hindbrain neurons,suggesting distinct roles in hindbrain morphogenesis.In summary,our study clarifies the specific role of RB1 in hindbrain neural cell proliferation and morphogenesis by regulating the E2f3–Gbx1 axis and the Hdac1–Gbx2 axis.These findings provide a research paradigm for exploring the differential proliferation of neurons in various brain regions.

关 键 词：ZEBRAFISH HINDBRAIN RB1 Gbx1/Gbx2 Proliferation 

分 类 号：R739.72[医药卫生—肿瘤]