生信分析结直肠癌差异表达免疫基因及其临床意义  

Bioinformatics analysis of differentially expressed immune genes in colorectal cancer and their clinical significance

作　　者：杨慧 穆晓峰 刘银 郝梦迪 丁磊 YANG Hui;MU Xiaofeng;LIU Yin;HAO Mengdi;DING Lei(Departmentof Oncology Radiotherapy,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038;Department of Oncology Surgery,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京世纪坛医院放疗科,北京100038 [2]首都医科大学附属北京世纪坛医院肿瘤外科,北京100038

出　　处：《生物技术》2024年第4期461-466,共6页Biotechnology

基　　金：国家自然科学基金项目(82170525)。

摘　　要：[目的]生物信息学分析结直肠癌(colorectal cancer,CRC)差异表达免疫基因(differentially expressed immune genes,DEIGs)及其临床意义,探究CRC的潜在治疗靶点。[方法]利用R软件筛选TCGA数据库中448例CRC患者和39例正常样本的差异表达基因(differentially expressed genes,DEGs)。从ImmPort数据库中获取免疫基因集,与DEGs取交集,获得DEIGs。对DEIGs进行GO和KEGG通路分析,探索其功能。将DEIGs与临床因素逐步纳入单因素Cox回归、Lasso回归、多因素Cox回归,探索DEIGs对CRC预后的影响。最后,分析DEIGs与免疫浸润的关系。[结果]共筛选到487个DEIGs,GO/KEGG分析显示,80个GO术语,74个KEGG术语被显著富集(P<0.05)。MC1R、UCN高表达,肿瘤分期晚、血管侵犯为CRC预后较差的独立危险因素(P<0.05)。MC1R、UCN高表达与CRC患者较差的总生存相关(P=0.00053、P=0.0012)。MC1R、UCN高低表达组中,T细胞、B细胞、NK细胞等浸润显著不同,差异有统计学意义(P<0.05)。[结论]生信分析发现2个DEIGs(MC1R、UCN)在CRC中高表达,与CRC预后较差及免疫细胞浸润显著相关,为后续研究奠定了基础。[Objective]To analyse differentially expressed immune genes(DEIGs)and their clinical significance of colorectal cancer(CRC)through bioinformatics,and to predict potential therapeutic targets for CRC.[Method]Differentially expressed genes(DEGs)between 448 CRC and 39 normal samples of TCGA were screened through the R software.Immune gene set was obtained from the ImmPort database.The intersection of DEGs and the immune gene set was taken to obtain DEIGs.GO and KEGG pathway analysis were performed to explore the functions of DEIGs.Then,the DEIGs and clinical factors were progressively incorporated into the univariate Cox regression,Lasso regression and multivariate Cox regression,to explore the impact of DEIGs on CRC prognosis.Finally,the relationship between the DEIGs and the immune infiltration was analyzed.[Result]A total of 487 DEIGs were identified,GO/KEGG analysis showed that 80 GO terms and 74 KEGG terms were significantly enriched(P<0.05).High expression of MC1R and UCN,tumor stage and vascular invasion were independent risk factors for poor prognosis of CRC(P<0.05).High expression of MC1R and UCN were associated with poorer overall survival(OS)in CRC patients(P=0.00053,P=0.0012).In the high and low expression groups of MC1R and UCN,the infiltration of T cells,B cells and NK cells showed significant differences,with statistical significance(P<0.05).[Conclusion]Based on bioinformatics,two DEIGs(MC1R and UCN)were found to be highly expressed in CRC,and they were significantly associated with the poor prognosis and immune cell infiltration of CRC,thus laying the foundation for subsequent research.

关 键 词：结直肠癌 生物信息学 TCGA 免疫基因 GO/KEGG分析 预后 MC1R UCN 

分 类 号：R735.3[医药卫生—肿瘤]