一阶导数光谱法测定染液组分浓度的实验教学设计  

Experimental Teaching Design for the Concentration Determination of Mixed Dyes by First Derivative Spectrophotometry

作　　者：刘慧青 徐中其 LIU Hui-Qing;XU Zhong-Qi(College of Chemistry and Chemical Engineering,Fundamental Experimental Chemistry Center,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学化学与化工学院,东华大学基础化学实验示范中心,上海201620

出　　处：《化学教育（中英文）》2024年第18期50-55,共6页Chinese Journal of Chemical Education

基　　金：国家先进印染技术创新中心科研基金项目(2022GCJJ15);东华大学校级本科一流课程建设项目(DHYLB-2021-14)。

摘　　要：针对缺乏定量分析染液浓度和分光光度法测定复杂体系实验的问题,在本科生仪器分析实验课程中,增设了一阶导数光谱法同时测定染液中三原色(活性红195、活性黄145和活性蓝19)浓度的实验。该实验分别选择655,566,326 nm作为活性蓝19、活性红195和活性黄145的检测波长,溶液pH为4~7,在10~100 mg/L范围内其一阶导数值与浓度呈良好的线性关系。对多组混合染液进行检测,通过测定值与实配值的对比,验证了该方法的精密度和准确性。本实验的实施可加深学生对光谱学理论知识的理解和应用,开拓学生在染料分析领域的视野,提高了学生对多组分的定量检测能力。To solve the lack of experiment classes,which are quantitative analysis of dye solutions and determination of complex systems by spectrophotometry,the simultaneous determination of dye components(reactive red 195,reactive yellow 145 and reactive blue 19)by first derivative spectrophotometry was designed as an instrumental analysis experiment course.655 nm,566 nm and 326 nm were respectively selected as detection wavelengths for reactive blue 19,reactive red 195 and reactive yellow 145,and the pH value of the solution is adjusted between 4 and 7.The methods had great linear relationships with the first derivative spectra in the concentration range of 10~100 mg/L.Many groups of mixed dye solution were determined,the comparison between measured and actual values verified the precision and accuracy of the method.The experiment deepened the students'understanding and applying of spectroscopy theoretical knowledge,expanded their vision in textile dyeing and printing analysis field,enhanced the ability to quantitatively analyze the multi-components.

关 键 词：一阶导数光谱法 活性三原色染料 同时测定 实验教学设计 

分 类 号：G642.423[文化科学—高等教育学] O657.3-4[文化科学—教育学] TQ610.7-4[理学—分析化学]