黄精渣水不溶性膳食纤维提取工艺优化及特性表征  

Extraction Process Optimization and Characterization of Water-Insoluble Dietary Fiber from Polygonatum sibiricum Residue

作　　者：王鑫 课净璇 王亚斌 王思琪 万鹏 马艳莉 卞华 陈志周[1] WANG Xin;KE Jingxuan;WANG Yabin;WANG Siqi;WAN Peng;MA Yanli;BIAN Hua;CHEN Zhizhou(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,China;Zhang Zhongjing College of Traditional Chinese Medicine,Nanyang Institute of Technology,Nanyang 473000,China)

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]南阳理工学院张仲景国医国药学院,河南南阳473000

出　　处：《保鲜与加工》2024年第9期53-64,共12页Storage and Process

基　　金：国家自然科学基金项目(32101960);河南省高等学校重点科研项目(23A550014);河南省张仲景方药与免疫调剂重点实验室开放课题(KFKT20221002);河南省高等学校青年骨干教师培养计划项目(2020GGJS226);南阳市协同创新重大专项(21XTCX12005);南阳理工学院交叉科学研究项目(23NGJY011);南阳市科技发展计划项目(23KJGG298)。

摘　　要：以废弃的黄精渣为原料,采用超声辅助酶法提取黄精渣水不溶性膳食纤维(PIDF)。通过单因素及响应面试验对提取工艺进行优化,并对提取的PIDF理化特性、结构特性表征及功能特性进行探究。结果表明,PIDF最佳提取条件为:超声时间28 min,超声功率230 W,料液比1∶24(g/mL),此时PIDF得率为86.88%±0.51%;最优提取工艺条件下所得的PIDF纤维素含量31.5%,半纤维素含量24.4%,木质素含量20.8%,持水力(12.47±0.31)g/g,持油力(8.03±0.24)g/g,膨胀力(8.03±0.19) mL/g,结合水力(7.80±0.51) g/g;PIDF葡萄糖吸附能力为(21 333±30.21)μmoL/g,总酚、总黄酮含量分别为(5.89±0.02) mg/g和(1.92±0.04) mg/g,抗氧化能力较佳;扫描电镜显示,PIDF表面为不规则片状,傅里叶变换红外光谱呈现明显的多糖特征吸收峰,X射线结果显示PIDF呈纤维素I晶型,符合膳食纤维结构。综上可知,黄精渣含有丰富的水不溶性膳食纤维,可作为良好的水不溶性膳食纤维的新型来源。研究结果为提高黄精副产物综合利用提供了理论依据。The water-insoluble dietary fiber(PIDF)was extracted by ultrasound-assisted enzymatic method from waste Polygonatum sibiricum residue.The extraction technology was optimized by single factor and response surface experiments.The physico-chemical characteristics,structural characterization and functional characteristics of the extracted PIDF were investigated.The results indicated that the optimal extraction conditions were as follows:ultr-asonic time 28 min,ultrasonic power 230 W,solid-liquid ratio 1:24(g/mL),and the PIDF yield was 86.88%±0.51%.The PIDF obtained under the optimal extraction condition contained 31.5% cellulose,24.4% hemicellulose,and 20.8% lignin.The water holding capacity of PIDF was(12.47±0.31)g/g,the oil holding capacity was(8.03±0.24)g/g,the swelling capacity was(8.03±0.19)mL/g,the combined hydraulic power was(7.80±0.51)g/g,the glucose adsorption capacity was(21333±30.21)μmoL/g,and the contents of total phenols and total flavonoids were(5.89±0.02)mg/g and(1.92+0.04)mg/g,respectively.PIDF had a better antioxidant capacity.Scanning electron microscope(SEM)showed that PIDF had an irregular sheet shape surface,and fourier transform infrared(FTIR)spectra showed an obvious polysaccharide characteristic absorption peak.X-ray results showed that PIDF had a cellulose I crystal structure,which was consistent with the structure of dietary fiber.In conclusion,Polygonatum sibiricum residue is rich in water-insoluble dietary fiber,which can be used as a new source of good water-insoluble dietary fiber.This study provide a theoretical basis for improving the comprehensive application of Polygonatum sibiricum by-products.

关 键 词：黄精渣 水不溶性膳食纤维 工艺优化 理化性质 

分 类 号：TS209[轻工技术与工程—食品科学]