Engineering W/O/W pickering emulsion for the enhanced antigen delivery and immune responses  

作　　者：Sihua Wu Xiaonan Huang Yin Ma Naoki Asakawa Yufei Xia 

机构地区：[1]State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100081,China [2]Key Laboratory of Biopharmaceutical Preparation and Delivery,Chinese Academy of Sciences,Beijing,100190,China [3]Division of Molecular Science,Graduate School of Science and Technology,Gunma University,1-5-1,Tenjin-cho,Kiryu,376-8515,Japan [4]Sinovac Biotech Ltd.,Beijing,100085,China [5]School of Chemical Engineering,Nanjing Tech University,Nanjing,211816,China [6]School of Chemical Engineering,University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Particuology》2024年第9期224-233,共10页颗粒学报（英文版）

基　　金：the National Key R&D Program of China(grant No.2022YFE0202500);Distinguished Young Scholars of the National Natural Science Foundation of China(grant No.T2222022);National Key Research and Development Program of China(grant No.2021YFC2302400,2021YFC2302600);Major Program of the National Natural Science Foundation of China(grant No.T2394503,T2394501);Key Project of the National Natural Science Foundation of China(grant No.32030062),CAS Project for。

摘　　要：Tumor immunotherapy,particularly cancer vaccines,holds promise for combating cancer by harnessing tailored immune responses against malignant cells.However,conventional approaches face challenges in efficiently delivering antigens for optimal immune activation.Emulsion adjuvants,like MF59,enhance cellular uptake but struggle to induce robust CD8^(+)T cell responses.Here,we introduce a novel strategy employing a water-in-oil-in-water(W/O/W)multiple Pickering emulsion(mPE)for antigen delivery.The mPE,utilizing biocompatible,pH-sensitive particles,encapsulates antigens within the inner water phase,ensuring enhanced intracellular processing and dictating the intracellular fate of antigens for improved cross-presentation.In vitro and in vivo studies demonstrated that mPEs induced robust dendritic cells activation and antigen cross-presentation,leading to a significantly enhanced immune response.Notably,calcium phosphate-stabilized mPE(CaP-mPE)illustrated the more robust IFN-γ^(+)T cell responses.In comparison with traditional surfactant-stabilized multiple emulsions,CaP-mPE significantly inhibit tumor growth and effectively prolong the survival of tumor-bearing mice.This innovative approach offers a promising avenue for the development of effective cancer vaccines with potent cellular immune responses.

关 键 词：Cancer vaccine Multiple pickeringemulsion ADJUVANT Antigen delivery Anti-tumor response 

分 类 号：R730.51[医药卫生—肿瘤]