αB-crystallin mini-peptides support corneal healing in vitro and in vivo in rabbit model  

作　　者：Namrata Maity Aditya Konar Sarbani Hazra 

机构地区：[1]Department of Veterinary Surgery&Radiology,West Bengal University of Animal and Fishery Sciences,Kolkata 700037,India [2]CSIR-IICB,Kolkata 700032,India

出　　处：《International Journal of Ophthalmology(English edition)》2024年第10期1772-1779,共8页国际眼科杂志（英文版）

基　　金：Supported by the DST Nano-mission,Govt of India,Grant No DST No.SR/NM/NS-1067/2016;Facilities were provided by the West Bengal University of Animal&Fishery Sciences and CSIR-IICB for conducting this research。

摘　　要：AIM:To evaluate if topical use of αB-crystallin minipeptides supports corneal healing following flap surgery.METHODS:Cultured corneal cells were treated with fluorescent taggedαB-crystallin mini-peptides to assess its internalization.Cultured corneal cells pre-treated with or without the mini-peptides were exposed to H_(2)O_(2) and cell viability was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Elongation of neurites of cultured trigeminal neurones was examined following treatment either withαB-crystallin mini-peptides or protein.Cultured trigeminal neurones were pre-treated either with αB-crystallin mini-peptides or crystallin protein and exposed to H_(2)O_(2) and presence of beading in the dendrites and axons was assessed.Corneal flap surgery was conducted on rabbit cornea and treated topically either withαB-crystallin peptide(0.5 mg/mL thrice daily for 14d)or phosphate-buffered saline(PBS).Corneal healing was evaluated under slit-lamp biomicroscope,mRNA expression of inflammatory cytokines were assessed and the corneas were evaluated by histopathology.RESULTS:Internalization ofαB-crystallin mini-peptides was ascertained by the detection of fluorescence within the corneal cells.The MTT assay revealed that treatment withαB-crystallin mini-peptide reduced cell death induced by H_(2)O_(2) treatment.The mini-peptides did not influence the elongation of trigeminal neurites,but significantly(P<0.05)reduced beading in the neurites.In rabbit eye,the treated corneas showed reduced hyper-reflective zones(P<0.05)and suppression in the expression of inflammatory cytokines.Histopathological examination also revealed reduction of inflammatory response in treated corneas.CONCLUSION:TheαB-crystallin mini-peptides restrict the damage to corneal cells and neurons and aids in corneal healing.

关 键 词：corneal healing αB-crystallin CYTOPROTECTION NEUROPROTECTION 

分 类 号：R772.2[医药卫生—眼科]