酸水解联合生物转化对剑麻皂素提取效率的影响  

Effects of the method of acid hydrolysis combining with biotransformationon tigogenin production efficiency in sisal

作　　者：温岚 朱作华[2] 陈伟男[3] 彭源德 谢纯良[1] WEN Lan;ZHU Zuohua;CHEN Weinan;PENG Yuande;XIE Chunliang(Institute of Applied Technology,Hunan Open University,Changsha,Hunan 410001,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China;Sisal and its Products quality supervision and Testing Center of MARA,Zhanjiang,Guangdong 524022,China)

机构地区：[1]湖南开放大学应用技术学院,湖南长沙410001 [2]中国农业科学院麻类研究所,湖南长沙410205 [3]农业农村部剑麻及制品质量监督检验测试中心,广东湛江524022

出　　处：《湖南农业大学学报（自然科学版）》2024年第4期111-118,共8页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：财政部、农业农村部国家麻类产业技术体系建设专项(CARS-16);中国农业科学院麻类研究所科技创新工程重大任务专项(CAAS-ASTIP-2023-IBFC)。

摘　　要：以剥制后的剑麻残渣为材料,筛选对其具有降解功能的菌株,并对菌株发酵条件进行优化,建立酸水解联合微生物发酵技术体系,以提升剑麻皂素提取率。结果表明:在剑麻渣中分离获得14个菌株,其中黑曲霉、哈茨木霉和里氏木霉对剑麻皂素具有较高的生物转化能力;对黑曲霉、哈茨木霉和里氏木霉进行发酵,发酵时间3 d,初始pH6、发酵温度30℃、添加体积分数0.1%的吐温-80时皂素提取率分别达到42.56、61.70、73.53 mg/g;在上述条件上,当添加1.0 mmol/L Fe^(2+)时,对黑曲霉和里氏木霉进行发酵,皂素提取率分别达到55.14、89.45 mg/g;当Fe^(2+)添加量为0.5 mmol/L,对哈茨木霉进行发酵,皂素提取率达到78.64 mg/g;单独酸水解试验结果表明,盐酸浓度为4.5 mol/L,水解时间为60 min时剑麻皂素提取率达到92.249 mg/g;采用1.5 mol/L盐酸结合黑曲霉、哈茨木霉和里氏木霉发酵对剑麻残渣进行提取,3个菌株提取剑麻皂素的提取率分别为78.70、95.40、107.3 mg/g。综上所述,采用酸水解联合微生物发酵法提取剑麻皂素,减少了2/3的酸消耗,且提高了剑麻皂素的提取率。Using the residue from decorticated sisal as a material,the bacterial strains with degradation capabilities were identified,and the fermentation conditions of the strains were optimized to establish a procedure of an acid hydrolysis combined with microbial fermentation method to improve the extraction rate of tigogenin.The results showed that 14 strains were isolated from sisal residue,among which Aspergillus niger,Trichoderma harzianum,and Trichoderma reeseihad a higherbiotransformation ability for tigogenin.Single-factor experiments on the fermentation conditions of the above three strains found that the tigogeninextraction rates reached 42.56,61.70,and 73.53 mg/g,respectively when the fermentation time was 3 days,the initial pH was 6,the fermentation temperature was 30°C and with 0.1%Tween-80addition.Withaddition ofFe^(2+)1.0 mmol/L,the tigogenin extraction rates of Aspergillus nigerand Trichoderma reeseireached 55.14 and 89.45 mg/g,respectively.And,with the additionof 0.5 mmol/LFe^(2+),the tigogeninextraction rate of Trichoderma harzianumreached 78.64 mg/g.Separate acid hydrolysis experiments showed that the extraction rate of tigogeninreached 92.249mg/gwith the condition of 4.5 mol/L hrdrohlorie acid and 60 minutes.Using 1.5 mol/L hydrochloric acid combined with the fermentation of Aspergillus niger,Trichoderma harzianum,and Trichoderma reesei,the extraction rates of tigogenin of the three strains were 78.70,95.40,and 107.3 mg/g,respectively.In summary,the acid hydrolysis combined with microbial fermentation method for extracting tigogeninreduced the acid consumption by 2/3.and increases the extraction rate of tigogenin.

关 键 词：剑麻 皂素 生物转化 酸水解 里氏木霉 

分 类 号：TQ460.38[化学工程—制药化工]