芦笋水提物的不同膜分离部位对二肽基肽酶-Ⅳ活性的影响及其成分分析  

Effect of Different Membrane Separation Parts on the Activity of Dipeptidyl Peptidase-IV in the Aqueous Extract of Asparagus officinalis and Its Compositional Analysis

作　　者：戚志强 田颖刚[1,2] QI Zhiqiang;TIAN Yinggang(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China;Engineering Research Center for Biomass Conversion,Ministry of Education,Nanchang University,Nanchang 330047,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,江西南昌330047 [2]南昌大学生物质转化教育部工程研究中心,江西南昌330047

出　　处：《食品工业科技》2024年第20期34-41,共8页Science and Technology of Food Industry

基　　金：运动-营养干预促进老年健康技术的建立(2020YFC2002902)。

摘　　要：为明确芦笋水提物中抑制二肽基肽酶-Ⅳ(Dipeptidyl peptidase-4,DPP-4)活性的主要功能成分,本文通过膜分离技术,制备了五种截留分子量不同的芦笋水提物部位,包括>30、10~30、6~10、3~6和<3 kDa,并对各部位进行了DPP-4抑制效果比较,对其总皂苷、总黄酮、总多糖、总多酚、总氮含量进行了比较分析。结果显示,总氮含量与抑制率呈最高正相关性(P<0.01),截留分子量大于30 kDa(UAJ1)是总氮含量最高的部位并表现出最强的抑制作用,其IC_(50)值为8.19 mg/mL,属于混合型抑制类型。总多糖含量与抑制率呈极显著正相关性,但次之(P<0.01)。总多酚含量与抑制率也呈显著的正相关性(P<0.05),而总黄酮含量与抑制率呈极显著负相关性(P<0.01)。总皂苷与抑制率之间无显著相关性。综合以上结果,芦笋水提物中对DPP-4起主要抑制作用的组分为分子量大于30 kDa的蛋白组分。这一发现可为天然DPP-4抑制剂在降血糖保健食品及药物开发提供参考。To identify the main functional components of asparagus aqueous extract that inhibit dipeptidyl peptidase-IV(DPP-4)activity,five asparagus aqueous extract parts with different molecular weight cut-offs,including>30,10~30,6~10,3~6,and<3 kDa,were successfully prepared by membrane separation technology in this study.The DPP-4 inhibition effect of each part was compared,and its total saponin,total flavonoid,total polysaccharide,total polyphenol,and total nitrogen contents were analysed.The results showed that the total nitrogen content showed the highest positive correlation with the inhibition rate(P<0.01),and the molecular weight cut-off(MWCO)greater than 30 kDa(UAJ1)was the site with the highest total nitrogen content and showed the strongest inhibition,with an IC50 value of 8.19 mg/mL,which was of the mixed inhibition type.Total polysaccharide content had the second highest positive correlation with the inhibition rate(P<0.01),total polyphenol content also showed a significant positive correlation with the inhibition rate(P<0.05),while total flavonoid content showed a very significant negative correlation with the inhibition rate(P<0.01).There was no significant correlation between total saponins and inhibition rate.In summary,the components in the aqueous extract of asparagus that exerted major inhibitory effects on DPP-4 were protein fractions with molecular weights greater than 30 kDa.This results would provide a reference for natural DPP-4 inhibitors in hypoglycaemic health food and drug development.

关 键 词：芦笋水提物 二肽基肽酶-Ⅳ 抑制作用 成分分析 

分 类 号：TS201.4[轻工技术与工程—食品科学]