松针提取液对茶树生长和虫害防治的影响机制  

Influence Mechanisms of Pine Needle Extracts on Tea Plant Growth and Pest Control

作　　者：张曼 黎健龙[1] 周波[1] 陈义勇 农红秋 崔莹莹 刘嘉裕 唐劲驰[1] ZHANG Man;LI Jianlong;ZHOU Bo;CHEN Yiyong;NONG Hongqiu;CUI Yingying;LIU Jiayu;TANG Jinchi(Tea Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization,Guangzhou 510640)

机构地区：[1]广东省农业科学院茶叶研究所/广东省茶树资源创新利用重点实验室,广州510640

出　　处：《中国农学通报》2024年第25期103-112,共10页Chinese Agricultural Science Bulletin

基　　金：广东省科技计划项目“韶关夏秋茶提质增效加工关键技术研究与示范”(2023B0202010018);国家现代农业(茶叶)产业技术体系建设专项“栽培生理与生态岗”(CARS-19);广东省农业科学院优博项目“春季茶树新梢萌发的调控基因及影响因素”(R2022YJ-YB3010);广东省农业科学院农业优势产业学科团队建设项目“茶园精准管控栽培”(202125TD)。

摘　　要：为探究松针提取液对茶树生长和虫害防治的影响机制,以喷施松针提取液和无菌水的茶树为材料进行比较实验,并进行转录组和代谢组学联合分析,解析其内在机制。结果表明,松针提取液能够促进茶树生长,提高茶树的发芽密度和叶片的叶绿素相对含量,提高茶叶产量,且不降低茶叶的品质。在这一过程中,AP2/ERF转录因子下调表达,乙烯信号途径发挥了重要的负调节作用。茶园喷施松针提取液不影响茶园节肢动物的群落结构,且对螨类和茶小绿叶蝉有一定的防治效果。转录组和代谢组联合分析表明,松针提取液提高茶树抗虫能力可能是由于激活了茶树的多酚类和磷脂类的代谢途径,相应代谢物质的含量显著提高,茶树抗氧化、清除自由基的能力提高,从而提高了茶树的抗逆性。研究结果可为天然植物抗逆性诱导剂的开发利用提供依据。In order to explore the effect of pine needle extracts on tea plant growth and pest control,tea trees treated with and without pine needle extracts were used as experiment materials,and the combined transcriptome and metabolome analysis was conducted to analyze the internal mechanism.The results showed that pine needle extracts could promote the growth of tea plants,with the increasing germination density,the relative content of chlorophyll in tea plant leaves,and the yield of tea without reducing the quality of tea.In this process,the expression of AP2/ERF transcription factors was suppressed,and ethylene signaling pathway played an important role of negative regulator.Besides,pine needle extracts treatments did not affect the community structure of arthropods of tea plants,and displayed a certain control effect on mites and tea green leafhoppers.The combined analysis of transcriptome and metabolome showed that the improvement of insect resistance of tea plant might be due to the activation of polyphenol and phospholipid metabolic pathways of tea plants,and the contents of corresponding metabolites were significantly increased.Thus,the antioxidant and free radical scavenging ability of tea plants were improved,so as to enhance the stress resistance of tea plants.In general,the results can provide a basis for the development and utilization of natural plant stress inducers.

关 键 词：松针提取液 茶树 乙烯信号通路 多酚类 抗氧化 虫害管理 转录组学 代谢组学 植物抗性诱导剂 

分 类 号：S571.1[农业科学—茶叶生产加工]