红托竹荪液体发酵培养基筛选及培养条件优化  

Screening of Liquid Fermentation Medium and culture condition for Dictyophora rubrovolvata

作　　者：江晓凌[1] 林辉[1] 杨驰[1] 肖冬来[1] 刘晓瑜 林衍铨[1] 马璐[1] Jiang Xiaoling;Lin Hui;Yang Chi;Xiao Donglai;Liu Xiaoyu;Lin Yanquan;Ma Lu(Institute of Edible Fungi,Fujian Academy of Agricultural Sciences/National and Local Joint Engineering Research Center for Breeding&Cultivation of Featured Edible Fungi,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院食用菌研究所/特色食用菌繁育与栽培国家地方联合工程研究中心,福建福州350013

出　　处：《东南园艺》2024年第4期278-284,共7页Southeast Horticulture

基　　金：福建省自然科学基金项目(2021J01504);福建省属公益类科研院所基本科研专项(2022R1035005);福建省农业科学院引导性科技创新项目(YDXM202209);福建省农业科学院科技创新团队(CXTD2021016-2)。

摘　　要：【目的】开展红托竹荪液体菌种发酵培养基的筛选,为红托竹荪液体菌种应用于工厂化栽培提供参考。【方法】以菌丝体生物量为指标,考察培养基营养物质及培养方式对红托竹荪液体发酵的影响。【结果】麦芽糖浆、玉米糖浆、玉米粉、发酵专用豆粕粉和黄豆粉可作为红托竹荪液体发酵培养基的适宜营养物质。当液体发酵培养基中玉米糖浆用量为20 g/L时,菌丝体生物量最大,为7.13 g/L。玉米粉、发酵专用豆粕粉、黄豆粉在供试范围内(0~4 g/L)可显著促进菌丝生长,当使用量为4g/L时,菌丝体生物量达到最大,分别为2.1 g/L、1.8 g/L、1.7 g/L。【结论】采用250 mL三角瓶,红托竹荪液体发酵的适宜培养条件为:培养基初始pH 5.5,装液量100~120 mL,接种量16%,培养时间14 d。【Objective】Screen the liquid spawn fermentation medium for Dictyophora rubrovolvata and provide references for the application of liquid spawn of D.rubrovolvata in industrialized cultivation.【Method】The effects of medium nutrients and culture methods on liquid fermentation of D.rubrovolvata were investigated with the mycelium biomass as the indicator.【Result】Maltose syrup,corn syrup,corn flour,soybean powder for fermentation and soybean flour are suitable for the liquid fermentation of D.rubrovolvata.When the amount of corn syrup was 20 g/L,the mycelial biomass was 7.13 g/L.Corn flour,soybean powder for fermentation and soybean flour could significantly promote the mycelial biomass in the test range,when the usage was 4 g/L,the mycelial biomass reach the maximum,which were 2.1 g/L,1.8 g/L,and 1.7 g/L respectively.【Conclusion】Using 250 mL Erlenmeyer flasks,the suitable culture conditions for D.rubrovolvata were listed as follows:initial medium pH 5.5,liquid volume 100-120 mL,inoculation volume 16%,and the terminal point of liquid spawn is 14 d.

关 键 词：红托竹荪 液体菌种 培养条件 

分 类 号：S646.8[农业科学—蔬菜学]