丹贝冻干粉对蛋白质营养不良小鼠的改善作用  

Freeze-Dried Tempeh Powder Ameliorates Protein Malnutrition in Mice

作　　者：王召君 王兆石 赵保民 董明盛[1] WANG Zhaojun;WANG Zhaoshi;ZHAO Baomin;DONG Mingsheng(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 230000,China;Jiangsu Pailezi Food Co.Ltd.,Xuzhou 221000,China)

机构地区：[1]南京农业大学食品科学技术学院,江苏南京230000 [2]江苏派乐滋食品有限公司,江苏徐州221000

出　　处：《食品科学》2024年第20期180-187,共8页Food Science

基　　金：江苏省农业科技自主创新项目(CX(21)2003)。

摘　　要：本实验以BALB/c蛋白质营养不良小鼠为模型,对其饲喂不同工艺处理的丹贝冻干粉进行饮食干预,并联合血生化参数分析、组织病理学和肠道菌群16S rDNA宏基因组学技术,研究丹贝冻干粉对蛋白营养不良小鼠的改善作用。结果表明,与低蛋白饲料饲喂组相比,饲喂丹贝冻干粉的蛋白质营养不良小鼠血清中的白蛋白、总蛋白、血红蛋白、胰岛素生长因子、血尿素氮和谷草转氨酶水平恢复正常,肠道菌群的均匀度、丰富度和多样性均得到改善,Lawsonibacter等有益菌相对丰度增加,多种基因的相对表达量提高,从而调控蛋白质代谢等生命过程。综上所述,丹贝冻干粉能显著改善小鼠蛋白质营养不良状况,其效果与等质量全价蛋白质(酪蛋白)相当。In this study,we assessed the ameliorative effect of dietary intervention with freeze-dried tempeh powder prepared using different processes on protein malnutrition in BALB/c mice by blood biochemical analysis,histopathological assays and intestinal microbiota 16S rDNA metagenomics technology.The results showed that compared with the low-protein diet feeding group,the levels of albumin,total protein,hemoglobin(HB),insulin growth factor-1(IGF-1),blood urea nitrogen(BUN)and aspartate aminotransferase(AST)in the serum of protein-malnourished mice fed freeze-dried tempeh powder returned to the normal level,the uniformity,richness and diversity of intestinal flora were improved,the relative abundance of beneficial bacteria such as Lawsonicacter increased,and the relative expression of a variety of genes increased,indicating that fed freeze-dried tempeh powder regulated life processes such as protein metabolism.In conclusion,freeze-dried tempeh powder can significantly ameliorate protein malnutrition in mice,and its effect is comparable to that of complete protein(casein)at the same mass.

关 键 词：蛋白质营养不良 丹贝 饮食干预 小鼠模型 肠道菌群 

分 类 号：TS201.4[轻工技术与工程—食品科学]