饭甑青冈叶绿体基因组及特征分析  

Chloroplast genome and characteristics analysis of Cyclobalanopsis fleuryi

作　　者：陆叶[1] 叶代全 翁禹豪 郑雪燕 李勇 刘鹏 Lu Ye;Ye Daiquan;WengYuhao;Zheng Xueyan;Li Yong;Liu Peng(Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education of China,Co-Innovation Center forSustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;National Germplasm Bank of Chinese fir at Fujian Yangkou Forest Farm,Nanping 353200,Chian;Fujian Jinshuo Biotechnology Co,Ltd,Sanming 253300,China)

机构地区：[1]南京林业大学林木遗传育种全国重点实验室、南方现代林业协同创新中心、南京林业大学林草学院,江苏南京210037 [2]福建省洋口林场国家杉木种质资源库,福建南平353200 [3]福建金硕生物科技有限公司,福建三明353300

出　　处：《江苏林业科技》2024年第4期1-7,共7页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省农业科技自主创新资金项目“耐盐型杂交鹅掌楸新品种培育及推广示范”(SCX(23)3628)。

摘　　要：饭甑青冈(Cyclobalanopsis fleuryi)是中国及越南的常绿树种,具有重要的经济和药用价值。该研究以饭甑青冈叶片为材料,通过Illumina HiSeq X ten高通量测序平台,对其叶绿体基因组进行测序、组装和注释。结果表明,饭甑青冈叶绿体全基因组具有典型的四分体结构,全长为160796 bp,共注释129个基因,其中包括83个蛋白编码基因、38个tRNA基因和8个rRNA基因。密码子偏好性分析表明饭甑青冈的密码子使用模式偏向以A或U结尾,亮氨酸(Leu)密码子使用频率最高,半胱氨酸(Cys)使用率最低。重复序列分析共检测到292个SSR位点,其中单碱基重复的SSR位点最多,有183个,无六碱基重复。壳斗科叶绿体基因组比较分析显示,反向重复区的位置和边界区域的基因分布无明显的收缩与扩张现象。系统发育分析表明,饭甑青冈与云山青冈(C.sessilifolia)、小叶青冈(C.myrsinifolia)亲缘关系密切。该研究为饭甑青冈的分子标记、物种鉴定、遗传多样性分析等研究提供了基础。Cyclobalanopsis fleuryi is an evergreen tree in south China and Vietnam with high economic and pharmaceutical values.In this study,the chloroplast genome of C.fleuryi was sequenced,assembled,and annotated using Illumina HiSeq X ten sequence platform.The results showed that the C.fleuryi chloroplast genome had a typical tetrad structure,with a total length of 160796 bp,which contained 129 genes,classified into 83 protein-coding genes,38 tRNA genes and 8 rRNA genes.Codon preference analysis revealed that the codon with A or U endings were preferred,with the highest frequency of Leu and the lowest frequency of Cys.In the chloroplast genomes,292 SSR loci were identified,with the highest number of single nucleotide repeats(183)and no hexanucleotide repeats.Comparison of the chloroplast genome among Fagaceae family showed that there were no obvious expansion or contraction at IR boundaries.The results of phylogenetic analysis indicated that C.fleuryi was closely related to C.sessilifolia and C.myrsinifolia.This study provides useful resources for molecular markers development,species identification,and genetic diversity analysis of C.fleuryi.

关 键 词：饭甑青冈 叶绿体基因组 密码子偏好性 壳斗科 系统发育分析 

分 类 号：Q786[生物学—分子生物学] S792.16[农业科学—林木遗传育种]