顺序式模拟移动床色谱高效纯化低聚异麦芽糖应用研究  

Application on efficient purification of isomaltooligosaccharides by sequential simulated moving bed chromatography

作　　者：阮圣慧 郑喜群 刘晓兰[4] 李良玉 姜彩霞 RUAN Shenghui;ZHENG Xiqun;LIU Xiaolan;LI Liangyu;JIANG Caixia(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;Engineering Research Center of Processing and Utilization of Grain By-products and Utilization of Ministry of Education,Daqing,Heilongjiang 163319,China;College of Food and Biological Engineering,Qiqihar University,Qiqihar,Heilongjiang 161006,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319 [3]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163319 [4]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006

出　　处：《食品与机械》2024年第7期163-168,230,共7页Food and Machinery

基　　金：黑龙江省“百千万”工程科技重大专项(编号:2019ZX06B02-1);黑龙江八一农垦大学研究生创新科研项目(编号:YJSCX2022-Y47)。

摘　　要：[目的]提高低聚糖行业的生产效率。[方法]以50型低聚异麦芽糖浆为原料,利用顺式模拟移动床色谱分离技术对其纯化。通过制备色谱单柱试验筛选出钾离子型树脂为合适的分离介质;根据物料平衡原理和顺序式模拟移动床基本原理近似确定分离工艺的初始操作参数,并对分离条件进行优化。[结果]最佳技术参数为:料液折光率60%、柱温65℃、进样量455 mL、进水量682 mL、进样流量37.4 mL/min、进水流量29.6 mL/min以及总循环流量364 mL/h,在此条件下低聚异麦芽糖出口折光为20.40%,纯度达到90.20%,收率达到85.50%。[结论]自主研制的顺序式模拟移动床色谱分离设备可用于高效纯化低聚异麦芽糖。[Objective]To improve the production efficiency of the oligosaccharide industry.[Methods]Isomaltooligosaccharides 50 syrup was used as the raw material,and purified by sequential simulated moving bed chromatography.Potassium ion resin was selected as the suitable separation medium by preparative chromatography single column experiment.Then,based onthe principle of material balance and the basic principle of sequential simulated moving bed,the initial operating parameters of separation process were approximately established.Further the separation conditions were optimized.[Results]The best technical parameters were as follows:feed refraction was 60%and column temperature was 65℃.At the same time quantity of flow in the entrance and eluent were 455 mL/h and 682 mL/h,respectively.The velocity of flow in the entrance and eluent were 37.4 mL/min and 29.6 mL/min,respectively.Besides,the circulating flow was kept within 364 mL/h.Finally,the outlet refraction of isomaltooligosaccharides was 20.40%with the purity of 90.20%and the yield of 85.50%.[Conclusion]The sequential simulated moving bed chromatographic separation equipment independently developed by the laboratory can efficiently purify isomaltooligosaccharides.

关 键 词：顺序式模拟移动床 色谱分离 纯化 低聚异麦芽糖 

分 类 号：TS201.2[轻工技术与工程—食品科学] O652.63[轻工技术与工程—食品科学与工程]