藜麦富硒萌发工艺优化及抗氧化活性研究  

Optimization of selenium-enriched germination process of quinoa and its antioxidant activity

作　　者：张玲 郑万财 兰永丽 张文刚 ZHANG Ling;ZHENG Wancai;LAN Yongli;ZHANG Wengang(Academy of Agriculture and Forestry Sciences,Qinghai University,Xining,Qinghai 810016,China;Key Laboratory of Qinghai Province Tibetan Plateau Agric-Product Processing,Xining,Qinghai 810016,China;Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources,Xining,Qinghai 810016,China;College of Food Science and Engineering,Northwest A&F University,Xianyang,Shaanxi 712100,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]青海省青藏高原农产品加工重点实验室,青海西宁810016 [3]青海省种质资源研究与利用实验室,青海西宁810016 [4]西北农林科技大学食品科学与工程学院,陕西咸阳712100

出　　处：《食品与机械》2024年第7期175-182,共8页Food and Machinery

基　　金：青海“昆仑英才·高端创新创业人才”计划特色人才项目(编号:2022-027)。

摘　　要：[目的]提高藜麦种子有机硒含量,开发优质富硒藜麦食品原料。[方法]以青白藜1号为原料,亚硒酸钠为硒源,通过单因素试验结合正交试验对藜麦富硒萌发工艺条件进行优化,进一步对富硒藜麦可溶性蛋白、多酚含量及抗氧化能力进行分析。[结果]藜麦富硒萌发最佳工艺条件为亚硒酸钠溶液质量浓度20 mg/L、浸泡温度25℃、浸泡时间6 h、浸泡液pH 7.0、萌发时间48 h、萌发温度20℃。该条件下,富硒萌发藜麦有机硒含量为349.52μg/kg。富硒萌发藜麦游离酚(236.35 mg/100 g)、结合酚(198.31 mg/100 g)含量最高,普通去离子水萌发藜麦可溶性蛋白(22.18%)含量最高。富硒萌发藜麦可溶性蛋白、游离酚和结合酚的抗氧化活性均最强,其次为普通去离子水萌发藜麦,未处理藜麦相对最低。[结论]富硒萌发能够显著提升藜麦有机硒、可溶性蛋白和多酚含量,并增强其抗氧化能力。[Objective]This study aimed to improve the organic selenium content of quinoa seeds and develop high-quality Se-rich quinoa food raw materials.[Methods]Using Qingbaili No.1 quinoa as raw material and sodium selenite as a selenium source,the selenium-enriched germination process conditions of quinoa were optimized through one-way experiments combined with orthogonal experiments.The soluble protein,polyphenol content,and antioxidant capacity of selenium-enriched quinoa were further analyzed.[Results]The optimal process conditions for selenium-enriched germination of quinoa were sodium selenite solution concentration of 20 mg/L,soaking temperature of 25℃,soaking time of 6 h,soaking solution pH of 7.0,germination time of 48 h,and germination temperature of 20℃.Under these conditions,the organic selenium content of selenium-enriched germinated quinoa was 349.52μg/kg.Selenium-enriched sprouted quinoa had the highest content of free phenols(236.35 mg/100 g)and bound phenols(198.31 mg/100 g),and control deionized water germinated quinoa had the highest content of soluble protein(22.18%).Notably,the antioxidant activities of soluble protein,free phenols,and bound phenols in selenium-rich germinated quinoa were the strongest,followed by those in control deionized water germinated quinoa,and untreated quinoa was relatively the lowest.[Conclusion]Se-enriched germination can significantly increase the contents of organic selenium,soluble protein and polyphenols,and enhance the antioxidant capacity of Quinoa.

关 键 词：藜麦 有机硒 萌发 工艺优化 抗氧化活性 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]