超声波辅助双水相法提取枸杞叶黄酮及其胰脂肪酶抑制活性  

Extraction of flavonoids from Goji(Lycium barbarum L.)leaves by ultrasonic⁃assisted two⁃phase aqueous method and the inhibitory activity of the extract on pancreatic lipase

作　　者：贾晓凤 高蝶 刘金旺 徐钰莹 范艳丽[1,2] JIA Xiaofeng;GAO Die;LIU Jinwang;XU Yuying;FAN Yanli(School of Food Science and Engineering,Ningxia University,Yinchuan,Ningxia 750021,China;Ningxia University Institute of Goji Berry Modern Industry,Yinchuan,Ningxia 750021,China)

机构地区：[1]宁夏大学食品科学与工程学院,宁夏银川750021 [2]宁夏大学枸杞现代产业学院,宁夏银川750021

出　　处：《食品与机械》2024年第8期144-151,共8页Food and Machinery

基　　金：国家级大学生创新创业计划项目(编号:202210749027)。

摘　　要：[目的]开发一种安全、无毒且高效的枸杞叶黄酮类化合物提取方法。[方法]采用超声波辅助双水相法实现高得率、绿色的枸杞叶黄酮类化合物提取,并评价产物活性。通过单因素和Plackett-Burman(PB)试验考察各因素对黄酮提取效果的影响,选取聚乙二醇(PEG400)使用量、硫酸铵使用量、料液比3个因素通过响应面中心组合试验设计优化提取条件;通过胰脂肪酶活性抑制试验评价提取物活性。[结果]超声波辅助双水相法提取枸杞叶黄酮最佳工艺条件为:聚乙二醇PEG相对分子质量400,PEG质量分数28%、硫酸铵质量分数16%、超声功率250 W,超声处理时间40 min、超声处理温度40℃、料液比1∶30 (g/g),该条件下所提取的枸杞叶黄酮提取物的总黄酮含量为(486.30±3.29) mg/g,其对胰脂肪酶活性具有显著抑制作用,半数抑制质量浓度(IC50)为1.06 mg/mL。[结论]最优提取条件下,超声波辅助双水相法所提取的枸杞叶黄酮得率高、溶剂残留低,该方法所得枸杞叶黄酮对胰脂肪酶具有良好的抑制作用。[Objective]This study aimed to achieve high yield and green extraction of flavonoids from Goji(Lycium barbarum L.)leaves using ultrasonic assisted aqueous two-phase method,and to evaluate the activity of extract.[Methods]Single factor and PB(Plackett⁃Burman)experiments were conducted to investigate the influence of various factors on the extraction efficiency of flavonoids.Three parameters were chosen to optimize the extraction conditions using a Central Composite Design(CCD)of the response surface experiment:the amount of polyethylene glycol(PEG400),the amount of ammonium sulfate,and the solid-liquid ratio.The extract's activity was assessed using an activity inhibition test of pancreatic lipase.[Results]The optimal process conditions for ultrasonic-assisted aqueous twophase extraction of flavonoids from Goji berry leaves were:28%polyethylene glycol 400,16%ammonium sulfate,ultrasonic power of 250 W,ultrasonic treatment time of 40 minutes,ultrasonic treatment temperature of 40℃,and solid-solid ratio of 1∶30(g/g).Under these conditions,the total flavonoid content of the extract was(486.30±3.29)mg/g,which had a significant inhibitory effect on pancreatic lipase activity,and the half inhibition concentration(IC50)was 1.06 mg/mL.[Conclusion]Under the optimal extraction conditions,the flavonoids extracted from L.barbarum leaves by ultrasonic-assisted aqueous phase method had high yield and low solvent residue,and the flavonoids from L.barbarum leaves obtained by this method had a good inhibitory effect on pancreatic lipase.

关 键 词：枸杞叶 黄酮 超声波 双水相法 胰脂肪酶 

分 类 号：TS201.2[轻工技术与工程—食品科学]