Metformin promotes anti-tumor immunity in STK11 mutant NSCLC through AXIN1-dependent upregulation of multiple nucleotide metabolites  

作　　者：ZHIGUO WANG KUNLIN LI CONGHUA LU MINGXIA FENG CAIYU LIN GUOFANG YIN DAN LUO WENYI LIU KAIYU JIN YUANYAO DOU DI WU JIE ZHENG KEJUN ZHANG LI LI XIANMING FAN 

机构地区：[1]Department of Respiratory and Critical Care Medicine,Affiliated Hospital of Southwest Medical University,Luzhou,646000,China [2]Department of Respiratory Disease,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042,China [3]Department of Trauma Medical Center,Daping Hospital,State Key Laboratory of Trauma,Burns and Combined Injury,Army Medical University,Chongqing,400042,China [4]Department of Respiratory Disease,People’s Hospital of Xuyong County,Luzhou,646000,China [5]Department of Outpatients,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042,China

出　　处：《Oncology Research》2024年第10期1637-1648,共12页肿瘤学研究（英文）

基　　金：People’s Hospital of Xuyong County-Southwest Medical University Science and Technology Strategic Cooperation Project(2023XYXNYD05);Guangdong Association of Clinical Trials(GACT)/Chinese Thoracic Oncology Group(CTONG)and Guangdong Provincial Key Lab of Translational Medicine in Lung Cancer(2017B030314120);Natural Science Foundation of Chongqing Municipality(CSTB2023NSCQ-MSX0554).

摘　　要：Background:Metformin has pleiotropic effects beyond glucose reduction,including tumor inhibition and immune regulation.It enhanced the anti-tumor effects of programmed cell death protein 1(PD-1)inhibitors in serine/threonine kinase 11(STK11)mutant non-small cell lung cancer(NSCLC)through an axis inhibition protein 1(AXIN1)-dependent manner.However,the alterations of tumor metabolism and metabolites upon metformin administration remain unclear.Methods:We performed untargeted metabolomics using liquid chromatography(LC)-mass spectrometry(MS)/MS system and conducted cell experiments to verify the results of bioinformatics analysis.Results:According to the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway database,most metabolites were annotated into metabolism,including nucleotide metabolism.Next,the differentially expressed metabolites in H460(refers to H460 cells),H460_met(refers to metformin-treated H460 cells),and H460_KO_met(refers to metformin-treated Axin1-/-H460 cells)were distributed into six clusters based on expression patterns.The clusters with a reversed expression pattern upon metformin treatment were selected for further analysis.We screened out metabolic pathways through KEGG pathway enrichment analysis and found that multiple nucleotide metabolites enriched in this pathway were upregulated.Furthermore,these metabolites enhanced the cytotoxicity of activated T cells on H460 cells in vitro and can activate the stimulator of the interferon genes(STING)pathway independently of AXIN1.Conclusion:Relying on AXIN1,metformin upregulated multiple nucleotide metabolites which promoted STING signaling and the killing of activated T cells in STK11 mutant NSCLC,indicating a potential immunotherapeutic strategy for STK11 mutant NSCLC.

关 键 词：METFORMIN Serine/threonine kinase 11(STK11) Lung cancer Axis inhibition protein 1(AXIN1) Nucleotide metabolites 

分 类 号：R734.2[医药卫生—肿瘤]