Transcriptome Dynamics and Cell Dialogs Between Oocytes and Granulosa Cells in Mouse Follicle Development  

作　　者：Wenju Liu Chuan Chen Yawei Gao Xinyu Cui Yuhan Zhang Liang Gu Yuanlin He Jing Li Shaorong Gao Rui Gao Cizhong Jiang 

机构地区：[1]Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education,Orthopaedic Department of Tongji Hospital,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China [2]Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital,Shanghai Key Laboratory of Signaling and Disease Research,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China [3]Frontier Science Center for Stem Cell Research,Tongji University,Shanghai 200092,China [4]Department of Epidemiology and Biostatistics,Jiangsu Key Lab of Cancer Biomarkers,Prevention and Treatment,Collaborative Innovation Center for Cancer Personalized Medicine,School of Public Health,Nanjing Medical University,Nanjing 210029,China

出　　处：《Genomics, Proteomics & Bioinformatics》2024年第2期93-106,共14页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the National Key R&D Program of China(Grant Nos.2019YFA0110000 and 2021YFA1100300);the National Natural Science Foundation of China(Grant Nos.31972882,31721003,31771419,and 31900621);the Natural Science Foundation of Shanghai Municipality,China(Grant No.21ZR1465500)。

摘　　要：The development and maturation of follicles is a sophisticated and multistage process.The dynamic gene expression of oocytes and their surrounding somatic cells and the dialogs between these cells are critical to this process.In this study,we accurately classified the oocyte and follicle development into nine stages and profiled the gene expression of mouse oocytes and their surrounding granulosa cells and cumulus cells.The clustering of the transcriptomes showed the trajectories of two distinct development courses of oocytes and their surrounding somatic cells.Gene expression changes precipitously increased at Type 4 stage and drastically dropped afterward within both oocytes and granulosa cells.Moreover,the number of differentially expressed genes between oocytes and granulosa cells dramatically increased at Type 4 stage,most of which persistently passed on to the later stages.Strikingly,cell communications within and between oocytes and granulosa cells became active from Type 4 stage onward.Cell dialogs connected oocytes and granulosa cells in both unidirectional and bidirectional manners.TGFB2/3,TGFBR2/3,INHBA/B,and ACVR1/1B/2B of TGF-βsignaling pathway functioned in the follicle development.NOTCH signaling pathway regulated the development of granulosa cells.Additionally,many maternally DNA methylation-or H3K27me3-imprinted genes remained active in granulosa cells but silent in oocytes during oogenesis.Collectively,Type 4 stage is the key turning point when significant transcription changes diverge the fate of oocytes and granulosa cells,and the cell dialogs become active to assure follicle development.These findings shed new insights on the transcriptome dynamics and cell dialogs facilitating the development and maturation of oocytes and follicles.

关 键 词：FOLLICULOGENESIS OOCYTE Granulosa cell TRANSCRIPTOME Cell dialog 

分 类 号：Q7[生物学—分子生物学]