多菌种发酵玉米蛋白粉饲料的制备工艺优化及抗氧化活性研究  

Study on the preparation process and antioxidant activities of multi-strain fermented corn gluten meal feed

作　　者：徐畅 郑喜群 刘晓兰[3] 韩国栋 王俊彤 李冠龙[3] XU Chang;ZHENG Xiqun;LIU Xiaolan;HAN Guodong;WANG Juntong;LI Guanlong(College of Food Science and Engineering,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang Province 163319,China;National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang Province 163319,China;College of Food and Biological Engineering,Qiqihar University,Qiqihar,Heilongjiang Province 161006,China;Engineering Research Center of Processing and Utilization of Grain By-products and Utilization of Ministry of Education,Daqing,Heilongjiang Province 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319 [3]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006 [4]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163319

出　　处：《中国饲料》2024年第19期172-181,共10页China Feed

基　　金：黑龙江省百千万工程科技重大专项(2020ZX06B01);中央支持地方高校改革发展资金高水平人才项目(2020GSP08)。

摘　　要：为提升玉米蛋白粉饲料的利用率,本研究以玉米蛋白粉和玉米胚芽粕为主要原料,根据乳酸菌:酵母菌:枯草芽孢杆菌=1:2:3的比例混合接种制备发酵饲料。以可溶性蛋白质含量和乳酸含量为指标,在单因素试验基础上,采用四因素三水平响应面试验设计,优化固态发酵工艺。结果表明:最佳发酵条件为发酵时间121.1 h、发酵温度36.1℃、含水量55.6%、玉米蛋白粉:玉米胚芽粕:糖蜜=7:2:1。在此条件下,发酵饲料中可溶蛋白含量为19.96%,乳酸含量为131.5 mmol/L。总氨基酸含量增加了13.04%,蛋白质二级结构α-螺旋与β-折叠比值由0.561提高到1.072,并且发酵饲料具有较好的抗氧化活性。In order to improve the utilization rate of corn protein meal feed,corn protein meal and corn germ meal were used as the main raw materials in this study to prepare fermented feed according to the ratio of lactic acid bacteria:yeast:Bacillus subtilis=1:2:3.Taking soluble protein content and lactic acid content as indicators,on the basis of single factor,the solid state fermentation process was optimized using four-factor three-level response surface design.The results showed that the optimum fermentation conditions were:fermentation time 121.1 h,fermentation temperature 36.1℃,water content 55.6%,corn gluten meal:corn germ meal:molasses=7:2:1.Under these conditions,the content of soluble protein and lactic acid in fermented feed was 19.96%and 131.5 mmol/L.Total amino acids increased by 13.04%,and protein secondary structure,α-Helix andβ-folding ratio increased from 0.561 to 1.072,and the fermented feed had good antioxidant activity.

关 键 词：玉米蛋白粉 可溶性蛋白质 抗氧化活性 氨基酸 

分 类 号：S816.6[农业科学—饲料科学]