茯砖茶调控脂噬干预小鼠非酒精性脂肪肝作用机制  

Regulation of Lipophagy by Fu Brick Tea to Intervene Non⁃Alcoholic Fatty Liver in Mice

作　　者：葛俊 刘富林[1] 喻长红 夏旭婷 李霞[1] 施敏 李琳莉 GE Jun;LIU Fulin;YU Changhong;XIA Xuting;LI Xia;SHI Min;LI Linli(Hunan University of Traditional Chinese Medicine,Changsha 410208,Hunan,China;Department of Spleen,Stomach,and Liver Diseases,First Traditional Chinese Medicine Hospital of Changde,Changde 415000,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]常德市第一中医医院脾胃肝病科,湖南常德415000

出　　处：《食品研究与开发》2024年第19期37-45,66,共10页Food Research and Development

基　　金：湖南省中医药科研课题项目(B2023151);湖南省自然科学基金项目(2022JJ30437);湖南省中医药科研计划项目(C2022033);湖南省教育厅科学研究项目(21A0232);常德市科技厅重点项目(2021SK055)

摘　　要：该文探讨茯砖茶调控脂噬干预高脂高糖饮食诱导的小鼠非酒精性脂肪肝的作用机制。将36只C57BL/6雄性小鼠随机分成对照组、模型组、阿托伐他汀组以及茯砖茶低、中、高剂量组。除对照组普通饲料喂养,其余5组适应性喂养1周后给予高脂高糖饮食喂养10周。阿托伐他汀组小鼠同时给予阿托伐他汀10 mg(/kg·d),茯砖茶低、中、高剂量组分别给予茯砖茶0.75、1.5、3.0 g(/kg·d),对照组和模型组给予生理盐水10 mL(/kg·d)。实验结束后取材,进行肝脏指数、血清肝功能、血脂、苏木精⁃伊红染色法(hematoxylin⁃eosin staining,HE)、油红O染色、透射电镜、免疫荧光双标、蛋白免疫印迹检测。结果表明,模型组小鼠肝组织病理学肝脏中出现了大量的脂肪空泡和严重的脂肪沉积,提示建模成功;茯砖茶低、中、高剂量组小鼠肝组织中脂肪空泡、脂肪沉积有不同程度减少,电镜下自噬溶酶体内被包裹物增加,肝脏病理损伤改善;肝脏指数、腹部脏器周围脂肪质量、门冬氨酸氨基转移酶(aspartate transami⁃nase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、胆固醇(cholesterol,CHO)、三酰甘油(triglycerides,TG)、低密度脂蛋白(low⁃density lipoprotein cholesterol,LDL⁃C)、核糖核酸结合蛋白62(sequestosome⁃1,p62)、磷酸化的磷脂酰肌醇3⁃激酶(phosphorylated phosphatidylinositol 3⁃kinase,p⁃PI3K)、磷酸化的蛋白激酶B(phosphorylated pro⁃tein kinase B,p⁃AKT)、磷酸化的哺乳动物雷帕霉素靶蛋白(phosphorylated mammalian target of rapamycin,p⁃mTOR)含量均有不同程度减少(P<0.05或P<0.01),高密度脂蛋白(high⁃density lipoprotein cholesterol,HDL⁃C)、溶酶体相关膜蛋白2(lysosome⁃associated membrane protein 2,LAMP2)、微管相关蛋白1轻链3⁃Ⅱ/微管相关蛋白1轻链3⁃I(mi⁃crotubule⁃associated protein1 light chain 3⁃Ⅱ/microtubule⁃associated protein1 light chain 3⁃I,LC3Ⅱ/LC3Ⅰ)的�This study explored the mechanism of Fu brick tea in regulating lipophagy for intervention in non-al-coholic fatty liver induced by high-fat and high-sugar diet in mice.Thirty-six male C57BL/6 micewere ran-domly divided into control,model,atorvastatin,and low-,medium-,and high-dose Fu brick tea groups.The control group was fed a normal diet,while the other five groups were fed a high-fat and high-sugar diet for 10 weeks after 1 week of acclimatization.The atorvastatin group received intragastric administration of atorvas-tatin at 10 mg/(kg·d),and the low-,medium-,and high-dose Fu brick tea groups received intragastric admin-istration of Fu brick tea at 0.75,1.5,3.0 g/(kg·d),respectively.The control and model groups received intra-gastric administration of saline at 10 mL/(kg·d).After 10 weeks of treatment,liver index,serum liver func-tion,and blood lipids were detected,and hematoxylin and eosin(HE)staining,oil red O staining,transmis-sion electron microscopy,immunofluorescence double-labeling,and western blot were performed.In the model group,extensive fatty vacuoles and severe lipid deposition were observed in liver tissues,indicating the successful establishment of the model.In the low-,medium-,and high-dose Fu brick tea groups,the extent of fatty vacuoles and lipid deposition in liver tissues decreased to varying degrees.The content encapsulated in autolysosome increased under electron microscopy,and liver pathological damage was improved.Liver index,abdominal fat mass,and the content of aspartate transaminase(AST),alanine aminotransferase(ALT),cho-lesterol(CHO),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),sequestosome-1(p62),phosphorylated phosphatidylinositol 3-kinase(p-PI3K),phosphorylated protein kinase B(p-AKT),and phos-phorylated mammalian target of rapamycin(p-mTOR)were reduced to different extent(P<0.05 or P<0.01),while lipoprotein cholesterol(HDL-C),lysosome-associated membrane protein 2(LAMP2),microtubule-as-sociated protein 1 light chain 3-II to microtubule-associated protein 1 li

关 键 词：非酒精性脂肪肝 茯砖茶 脂噬 脂滴 PI3K/AKT/mTOR通路 

分 类 号：TS272.54[农业科学—茶叶生产加工] R575.5[轻工技术与工程—农产品加工及贮藏工程]