补骨脂糖蛋白提取工艺优化及成分测定  

Optimization of Extraction Process and Component Determination of Psoralea corylifolia Glycoprotein

作　　者：辛加敏 阮婧华 王聪 谭荣 查鑫 李广松 王海燕 韦利芬 胡小伟 XIN Jiamin;RUAN Jinghua;WANG Cong;TAN Rong;ZHA Xin;LI Guangsong;WANG Haiyan;WEI Lifen;HU Xiaowei(The First Affiliated Hospital of Guizhou University of Tradition Chinese Medicine,Guiyang 550001,Guizhou,China;Guizhou University of Tradition Chinese Medicine,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州中医药大学第一附属医院,贵州贵阳550001 [2]贵州中医药大学,贵州贵阳550025

出　　处：《食品研究与开发》2024年第19期97-105,共9页Food Research and Development

基　　金：国家自然科学基金项目(82060643);贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2023-105);贵州省科技计划项目(黔科合平台人才[2021]5621号)。

摘　　要：为优化补骨脂糖蛋白(Psoralea corylifolia glycoprotein,PCG)提取工艺,并对PCG中成分进行分析,在单因素试验基础上,以PCG提取率为指标,对料液比、酶解时间、加酶量进行考察,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定PCG分子量,采用红外光谱仪、超高速全自动氨基酸测定分析仪、高效液相色谱仪测定PCG成分。结果表明,碱性蛋白酶对PCG提取率最高,优化后PCG的提取工艺为料液比为1∶30(g/mL)、酶解时间2.5h、加酶量3000U/g,提取得到的PCG分子量为17~75 kDa。PCG中含有氨基酸和糖类基团,氨基酸种类为16种,谷氨酸含量最高,为5.13%,单糖种类为5种,甘露糖含量最高,为44.43%。In this study,measures were taken to optimize the extraction process of Psoralea corylifolia glyco-protein(PCG),and to analyze its components.On the basis of single-factor experiment,PCG extraction rate was taken as the indicator to examine material liquid ratio,enzymatic hydrolysis time,and the amount of en-zyme added.PCG's molecular weight was determined by sodium dodecyl sulfate polyacrylamide gel electropho-resis(SDS-PAGE).PCG's components were determined by Fourier transform infrared spectroscopy(FTIR),ultra-high-speed automatic amino acid analyzer,and high performance liquid chromatography(HPLC).The results showed that the PCG extraction rate was the highest with the addition of alkaline protease.The opti-mized extraction process for PCG was as follows:material liquid ratio was 1∶30(g/mL),enzymatic hydrolysis time was 2.5 h,and the amount of enzyme added was 3000 U/g.The molecular weight of PCG extracted in the optimized process was 17-75 kDa.PCG contained amino acid and carbohydrate groups.Among 16 types of amino acids in the PCG,the content of glutamic acid was at 5.13%,the highest in all.Besides,there were 5 types of monosaccharides,with the content of mannose at 44.43%,the highest in all.

关 键 词：补骨脂糖蛋白 碱性蛋白酶 提取工艺 氨基酸 单糖 

分 类 号：TS201.21[轻工技术与工程—食品科学]