酶膜固定技术在食品功效成分制备中的应用  

Enzyme-Membrane Immobilization Technology and Its Application for Fabrication of Food Functional Component

作　　者：汪芳 周雷 陈哲[2] 罗建泉 WANG Fang;ZHOU Lei;CHEN Zhe;LUO Jianquan(College of Food and Biotechnology,Wuhan Institute of Design and Science,Wuhan 430205,China;School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]武汉设计工程学院食品与生物科技学院,湖北武汉430205 [2]武汉轻工大学硒科学与工程现代产业学院,湖北武汉430023 [3]生化工程国家重点实验室中国科学院过程工程研究所,北京100190

出　　处：《食品科技》2024年第8期57-63,共7页Food Science and Technology

基　　金：湖北省自然科学基金项目(2024AFC027);湖北省高校优秀中青年科技创新团队项目(T2020012)。

摘　　要：将生物酶通过物理化学技术固定到分离膜表面构建酶膜反应器,可以在一次操作中同步进行酶解反应和产物分离。由于酶使用效率高、节约能耗、废水排放少等优点,酶膜固定技术被广泛应用于食品领域。文章介绍了包括物理吸附/包埋、共价结合、交联等酶膜固定化技术,归纳了提高酶固定化性能的方法,综述了酶膜固定技术在多肽制备、功能性糖制备、油脂水解与合成和多酚酯化等功效成分制备中的应用,并针对酶膜固定技术发展现状提出了进一步展望,旨在为深入研究开发契合食品工程应用场景的高效酶膜固定技术提供理论和技术支持。Enzymatic membrane reactor,constructed by immobilizing enzymes on membranes via physiochemical treatments,can carry out enzymatic reaction and product separation in one step operation.Due to the advantages,such as high efficiency of enzyme,low consumption of energy and less discharge of wastewater,enzyme-membrane technology has been widely applied in food technology.This work introduced enzyme immobilization technologies including physical adsorption/embedding,covalent binding,and cross-linking,as well as methods to improve the immobilization efficiency.The applications of enzyme-membrane immobilization technology on peptide preparation,functional polysaccharides production,lipid hydrolysis and synthesis,and polyphenol esterification were also summarized.Further prospects for the development of enzyme-membrane immobilization technology was proposed.The aim is to provide theoretical and technical support for further research and development of efficient enzyme-membrane immobilization technology suitable for food engineering application scenarios.

关 键 词：酶膜固定 酶膜反应器 食品功效成分 

分 类 号：TS201.25[轻工技术与工程—食品科学]