实时荧光PCR法检测鸭血制品中其他动物源性成分  

Qualitative Detection Methods of Animal Derived Ingredients in Duck Blood by Real-Time Fluorescence PCR

作　　者：邓迎春 刘颖 王一凡 郭旭光 苏利霞 陶健 DENG Yingchun;LIU Ying;WANG Yifan;GUO Xuguang;SU Lixia;TAO Jian(Henan Institute of Food and Salt Industry Inspection Technology,Zhengzhou 450003,China;Food Safety and Emergency Support Center of Henan Provincial Administration for Market Regulation,Zhengzhou 450003,China)

机构地区：[1]河南省食品和盐业检验技术研究院,河南郑州450003 [2]河南省市场监督管理局食品安全和应急保障中心,河南郑州450003

出　　处：《食品科技》2024年第8期342-348,共7页Food Science and Technology

基　　金：河南省市场局科技项目(HNSCJGK202490);河南省科技计划项目(222102410011)。

摘　　要：目的:为对市售鸭血制品进行掺假检测,文章建立了实时荧光聚合酶链式反应法(Polymerase chain reaction,PCR)检测鸭血中鸡、鸭、鹅、猪、牛、羊等动物源性成分的定性检验方法。方法:考察了3种不同的DNA提取方式对检测结果的影响,确定了方法的检出限,并对抽取的51份市售鸭血样品进行检测。结果:烘干称质量提取的DNA浓度及扩增效率最高,该方法灵敏度可达到1%。51份实际样本中共发现8批样本存在掺假行为。结论:该方法可实现对鸭血中其他动物源性成分的定性检测,为执法监管部门提供有力的技术保障。Objective:In order to detect the adulteration of animal derived ingredients in duck blood products,a qualitative detection method was developed in this paper for real-time fluorescence polymerase chain reaction(PCR)of animal derived components such as chicken,duck,goose,pig,cow,and sheep in duck blood.Method:The method investigated the impact of three different DNA extraction methods on the results,explored the detection limit of the method,and tested 51 duck blood samples.Results:The DNA concentration and amplification efficiency extracted by drying and weighing were the highest,and the sensitivity of the method could reach 1%.In actual samples detection,8 out of 51 duck samples were found to have other animal derived ingredients.Conclusion:This method realize the qualitative detection of other animal derived components in duck blood,providing strong technical support for law enforcement and regulatory authorities.

关 键 词：实时荧光聚合酶链式反应法 鸭血 DNA提取 动物源性成分 

分 类 号：TS207.3[轻工技术与工程—食品科学]