镧、铈、镨和钕对生物膜群落胞外酶的联合毒性效应  

Combined toxic effects of La,Ce,Pr,and Nd on biofilm extracellular enzymes

作　　者：王雪梅 杨冬萍[1] 胡海燕 彭聪 胡金朝 WANG Xuemei;YANG Dongping;HU Haiyan;PENG Cong;HU Jinzhao(College of Resources and Environment,Xichang University,Xichang 615013,China;State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil&Water Pollution,Chengdu University of Technology,Chengdu 610059,China)

机构地区：[1]西昌学院资源与环境学院,西昌615013 [2]成都理工大学国家环境保护水土污染协同控制与联合修复重点实验室,成都610059

出　　处：《应用与环境生物学报》2024年第4期693-698,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(41967033);凉山州科技计划重点研发项目(21ZDYF0115)资助。

摘　　要：稀土元素作为新兴污染物,其水生态毒性受到广泛关注.现有稀土的毒理学研究多集中于单个稀土元素的毒性效应,稀土元素混合物对水生生物的影响尚缺乏深入报道.采用单因素毒性试验、析因设计联合毒性试验和二元混合毒性试验的方法,研究镧(La)、铈(Ce)、镨(Pr)和钕(Nd)对生物膜群落β-葡萄糖苷酶(BETA)活性的毒性效应.结果表明,单一元素在0.5-10 mg/L的范围内对BETA活性的影响表现为“低促高抑”,其中Pr的毒性作用最强,5 mg/L时抑制率最高.析因设计试验结果的方差分析和多元线性回归表明Nd对联合毒性产生负的影响,La对联合促进产生负的影响,三元和四元交互作用对联合毒性的贡献不显著.二元混合验证实验表明La*Ce在高低两种水平均表现为促进作用,Ce*Pr、Ce*Nd、Pr*Nd均为低促高抑作用.La*Ce、Ce*Pr、Pr*Nd低浓度的二元组合表现为协同作用,高浓度为拮抗作用.Ce*Nd的交互作用不受浓度影响,均表现为拮抗作用.本研究表明稀土的联合毒性效应及交互作用模式同时受元素性质和浓度组合的影响,可通过提高Ce*Pr、Ce*Nd、Pr*Nd的拮抗作用来降低稀土的联合毒性,这对于减少稀土复合污染将有一定意义.(图3表4参35)The aquatic ecological toxicity of rare earth elements(REEs),considered emerging pollutants,has garnered significant attention.Existing toxicological research has primarily focused on the individual toxic effects of REEs,with a lack of comprehensive investigations into the impacts of mixtures of these elements on aquatic organisms.We conducted single-factor toxicity,factorial design combined toxicity,and binary mixture toxicity experiments to investigate the toxic effects of La,Ce,Pr,and Nd on the β-glucosidase(BETA)activity in biofilms.Results showed that the impact of individual elements on BETA activity exhibited a‘low-stimulation,high-inhibition’pattern in the range of 0.5-10 mg/L,with Pr demonstrating the strongest toxic effect at 5 mg/L.Analysis of variance and multiple linear regressions indicated that Nd had a negative effect on combined toxicity,whereas La had a negative effect on combined stimulation.The contribution of ternary and quaternary interactions to combined toxicity was not significant.A binary mixed validation experiment demonstrated that the La*Ce combination had a promoting effect at both high and low levels,whereas Ce*Pr,Ce*Nd,and Pr*Nd exhibited low promoting and high inhibiting effects.The binary combinations of low La*Ce,Ce*Pr,and Pr*Nd concentrations displayed synergistic effects,while high concentrations showed antagonistic effects.The Ce*Nd interaction was unaffected by concentration and exhibited antagonistic effects.In conclusion,the combined toxic effects and interaction modes of rare earths are influenced by both elemental properties and concentration combinations.Investigating the antagonistic effects of Ce*Pr,Ce*Nd,and Pr*Nd is crucial for mitigating the collective toxicity of rare earths.This research is of considerable importance for reducing rare earth composite pollution.

关 键 词：稀土元素 生物膜群落 Β-葡萄糖苷酶 联合毒性 析因设计 

分 类 号：X171.5[环境科学与工程—环境科学]