基于黄棪(Camellia sinensis)基因组的DXS基因家族鉴定及表达  

Genome-wide identification and expression of the DXS gene family in Huangdan(Camellia sinensis)

作　　者：曾珊珊 高婷 谷梦雅 侯炳豪 任卫威 张宇航 叶乃兴[1] ZENG Shanshan;GAO Ting;GU Mengya;HOU Binghao;REN Weiwei;ZHANG Yuhang;YE Naixing(College of Horticulture,Fujian Agriculture and Forestry University/Key Laboratory of Ministry of Education for Genetics,Breeding,and Multiple Utilization of Crops,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺学院/作物遗传育种与综合利用教育部重点实验室,福州350002

出　　处：《应用与环境生物学报》2024年第4期811-817,共7页Chinese Journal of Applied and Environmental Biology

基　　金：安溪茶叶重大科技创新专项项目(AX2021001);中国乌龙茶产业协同创新中心专项(闽教科[2015]75号);福建张天福茶叶发展基金会科技创新基金项目(FJZTF01)资助。

摘　　要：1-脱氧-D-木酮糖-5-磷酸合成酶(DXS)是MEP途径的第一个限速酶,在茶树抗性的形成中起到重要作用.为探究茶树DXS基因家族的潜在功能,利用生物信息学和实时荧光定量PCR技术,对茶树DXS基因家族进行鉴定及表达分析.采用生物信息学方法,从‘黄棪’和‘铁观音’茶树中分别鉴定出5个DXS基因家族成员,分别命名为HD-CsDXS1-CsDXS5和TGY-CsDXS1-CsDXS5,系统发育结果表明其分属3个亚家族,相同亚家族的CsDXS基因外显子-内含子数目及排列顺序基本一致.CsDXS启动子上存在众多与植物发育、激素和胁迫响应相关的顺式作用元件.荧光定量检测发现,HD-CsDXS2和HD-CsDXS5在MeJA、GA、ABA、低温和干旱处理下表达量均显著上调,HD-CsDXS1和HD-CsDXS4在GA、ABA和干旱处理下表达量显著上调,HD-CsDXS3仅在GA和干旱处理下表达量上调.本研究表明CsDXS在非生物胁迫下均被诱导表达,可能与茶树抗逆性密切相关,结果可为进一步探究茶树DXS基因家族的功能研究提供参考.(图8表2参30)1-Deoxy-D-xylulose-5-phosphate synthetase(DXS)is the first rate-limiting enzyme in the methylerythritol-4-phosphate(MEP)pathway of plant terpene synthesis,playing a crucial role in tea plant resistance.To investigate the potential functions of the DXS gene family,we employed bioinformatics analysis and quantitative reverse transcription PCR(qRT-PCR)to identify and analyze the expression patterns of the CsDXS gene family.Five DXS gene family members were identified from Huangdan and five from Tieguanyin tea plants,named HD-CsDXS1–5 and TGY-CsDXS1-5,respectively.Phylogenetic analysis categorized them into three subfamilies,with consistent exon-intron structure within each subfamily.Promoter analysis revealed numerous cis-acting elements associated with stress responses,hormones,and growth.Quantitative fluorescence detection indicated significant upregulation of HD-CsDXS2 and HD-CsDXS5 under methyl jasmonate(MeJA),gibberellin(GA),abscisic acid(ABA),low temperature,and drought treatments;HD-CsDXS1 and HD-CsDXS4 were significantly upregulated under GA,ABA,and drought treatments;and HD-CsDXS3 was upregulated only under GA and drought treatments.These findings suggest that CsDXS genes are induced under abiotic stress and may contribute to the stress resistance of tea plants.Our results provide a foundation for further research on the functions of the DXS gene family in tea plants.

关 键 词：茶树 1-脱氧-D-木酮糖-5-磷酸合成酶 非生物胁迫 表达分析 

分 类 号：Q943.2[生物学—植物学] S571.1[农业科学—茶叶生产加工]