高效液相色谱法检测软糖中褪黑素含量  

Determination of Melatonin Content in Soft Candy by High Performance Liquid Chromatography

作　　者：林丽炫 张丽贤 林岱滨 LIN Lixuan;ZHANG Lixian;LIN Daibin(Guangdong Yichao Biological Co.,Ltd.,Shantou 515000,China)

机构地区：[1]广东亿超生物科技有限公司,广东汕头515000

出　　处：《食品安全导刊》2024年第26期59-61,共3页China Food Safety Magazine

摘　　要：目的:建立高效液相色谱法(High Performance Liquid Chromatography,HPLC)检测软糖产品中褪黑素含量的方法。方法:软糖样品经液氮冷冻粉碎后,加0.5 g·L^(-1)磷酸二氢钾溶液提取,乙腈定容,HPLC分析,外标法定量。结果:在0.08~0.20 mg·mL^(-1)的浓度范围内,方法的线性良好,线性相关系数为0.9999;方法的平均加标回收率为97.84%~100.32%,精密度为0.24%~0.41%。结论:该方法专属性良好,操作简单,结果准确,适用于含有褪黑素的软糖产品的检测。Objective:To establish a method for detecting melatonin in soft candy products by high performance liquid chromatography(HPLC).Method:The soft candy samples were frozen and crushed with liquid nitrogen,extracted with 0.5 g·L^(-1)potassium dihydrogen phosphate solution,fixed to volume with acetonitrile,analyzed by HPLC,and quantified by external standard method.Result:In the concentration range of 0.08~0.20 mg·mL^(-1),the method had good linearity,with a linear correlation coefficient of 0.9999;the average spike recovery of the method was 97.84%~100.32%,and the precision was 0.24%~0.41%.Conclusion:This method has good specificity,simple operation,accurate results,and is suitable for the detection of soft candy products containing melatonin.

关 键 词：褪黑素 含量测定 方法研究 

分 类 号：TS246.56[轻工技术与工程—制糖工程] O657.72[轻工技术与工程—食品科学与工程]