玉米赤霉烯酮降解酶热稳定性及底物特异性改造策略  

Strategies for Thermostability and Substrate Specific Modification of Zearalenone Degrading Enzymes

作　　者：秦立晔 刘晓萌 王寿南 张晓琳 印铁 任顺成[1] 仉磊 Qin Liye;Liu Xiaomeng;Wang Shounan;Zhang Xiaolin;Yin Tie;Ren Shuncheng;Zhang Lei(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001;COFCO Nutrition and Health Research Institute,Beijing Engineering Research Center of Livestock Product Quality and Safety Source Control,Beijing 102209;College of Food Science and Engineering,Nanjing University of Finance and Economics,Collaborative Innovation Center for Modern Grain Circulation and Safety,Nanjing 210023)

机构地区：[1]河南工业大学粮油食品学院,郑州450001 [2]中粮营养健康研究院,北京市畜产品质量安全源头控制工程技术研究中心,北京102209 [3]南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,南京210023

出　　处：《中国粮油学报》2024年第8期42-51,共10页Journal of the Chinese Cereals and Oils Association

基　　金：“十四五”国家重点研发计划项目(2021YFC2103003)。

摘　　要：玉米赤霉烯酮(zearalenone,ZEN)及其衍生物是污染范围较大的真菌毒素,广泛存在于玉米、大麦、小麦和高粱等谷物及其副产品中,对饲料行业和畜牧养殖业造成严重的经济损失。利用生物技术对ZEN及其衍生物进行降解和脱除是未来解决ZEN污染的重要途径。然而,目前发现的ZEN降解酶热稳定性差,催化效率低,难以满足工业生产中高温、酸、碱等极端条件的需求。本文综述了近年来利用蛋白质工程提升ZEN降解酶热稳定性和底物特异性的改造策略,阐明ZEN降解酶改造的研究进展及存在问题,为开发ZEN降解酶的工程改造提供参考。Zearalenone(Zen)and its derivatives are one of the mycotoxins with a wide range of contamination,and widely exist grains and their byproducts,(such as corn,barley,wheat,sorghum et al.),posing a serious threat to animal and human health.The degradation and removal of ZEN and its derivatives by biotechnology will be the major method to solve the pollution of ZEN in the future.However,it is currently known that ZEN degrading enzyme has poor thermal stability and low catalytic efficiency,and difficult to meet the requirements of industrial production.In this paper,the modification strategies with protein engineering for improving the thermal stability and substrate specificity of ZEN degrading enzymes were reviewed,and the research advance and problems of the ZEN degrading enzymes were elucidated to provide reference for the development of engineering modification of ZEN degrading enzymes.

关 键 词：玉米赤霉烯酮 降解酶 热稳定性 底物特异性 分子改造 

分 类 号：Q814[生物学—生物工程]