芥菜型油菜类黄酮对UC大鼠结肠黏膜的干预机制  

Intervention mechanism of flavonoids from Brassica juncea on colonic mucosa of UC rats

作　　者：余欣 贾云娇 陈慧珠 唐宇琦 王慧[1] YU Xin;JIA Yunjiao;CHEN Huizhu;TANG Yuqi;WANG Hui(Qiqihar Medical University,Heilongjiang,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《中国医药科学》2024年第18期10-13,共4页China Medicine And Pharmacy

基　　金：黑龙江省大学生创新创业训练项目(S202211230018)。

摘　　要：目的探究芥菜型油菜类黄酮对溃疡性结肠炎(UC)大鼠结肠黏膜干预机制。方法将45只SD大鼠分为空白组(10只)和造模组(35只),造模组按随机数表法分为模型组、芥菜型油菜类黄酮低、高浓度组,给药治疗后,进行疾病活动指数(DAI)评分;结肠黏膜损伤指数(CMDI)评分;结肠组织病理学检查;酶联免疫法检测大鼠结肠黏膜肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-10的含量;免疫组化法检测黏蛋白2(MUC2)和表皮生长因子(EGFR)的表达。结果与模型组比较,芥菜型油菜类黄酮低、高浓度组DAI评分、CDMI评分、TNF-α、IL-1β含量均降低(P<0.05),IL-10含量及MUC2、EGFR表达水平升高(P<0.05);与低浓度组比较,高浓度组DAI评分、CDMI评分、TNF-α、IL-1β含量降低(P<0.05),IL-10含量及MUC2、EGFR表达水平升高(P<0.05)。HE染色结果显示低、高浓度组大鼠的结肠组织病变程度均有所改善,病理学损伤结果均降低,高浓度组降低更明显。结论芥菜型油菜类黄酮可以通过调节UC大鼠结肠黏膜TNF-α、IL-1β、IL-10的水平,增加MUC2、EGFR蛋白表达来调控肠上皮系统,从而达到修复结肠黏膜的目的。Objective To explore the intervention mechanism of flavonoids from Brassica juncea on colonic mucosa in ulcerative colitis(UC)rats.Methods A total of 45 SD rats were divided into a blank group(10 rats)and a modeling group(35 rats).The modeling group was randomly divided into a model group,a low and a high concentration group of flavonoids in Brassica juncea according to random number table.After treatment,the disease activity index(DAI)score,colonmucosa damage index(CMDI)score,colonic tissue pathology examination were performed;and the contents of tumor necrosis factor(TNF)-α,interleukin(IL)-1βand IL-10 in colonic mucosa of rats were detected by enzymelinked immunosorbent assay,and the expressions of mucin 2(MUC2)and epidermal growth factor(EGFR)were detected by immunohistochemistry.Results Compared with model group,the DAI score,CDMI score,and the contents of TNF-αand IL-1βof the low and the high concentration group of flavonoids in Brassica juncea decreased(P<0.05),while the content of IL-10,the expression levels of MUC2 and EGFR increased(P<0.05).Compared with the low concentration group,the DAI score,CDMI score,and the contents of TNF-αand IL-1βdecreased in high concentration group(P<0.05),while the content of IL-10,the expression levels of MUC2 and EGFR increased in high concentration group(P<0.05).HE staining results showed that the degree of colonic tissue lesions of rats in both low and high concentration groups were improved and the pathological damage results were reduced,especially in high concentration group.Conclusion Flavonoids from Brassica juncea can regulate the intestinal epithelial system by regulating the levels of TNF-α,IL-1βand IL-10 in the colonic mucosa of UC rats and increasing the expression of MUC2 and EGFR proteins,so as to achieve the purpose of repairing the colonic mucosa.

关 键 词：芥菜型油菜 类黄酮 溃疡性结肠炎 黏蛋白2 表皮生长因子 

分 类 号：R574.6[医药卫生—消化系统]