茯苓酸对冈田酸诱导PC12细胞损伤的保护作用  

Protective Effect of Pachymic Acid on Okadaic Acid-induced Neurotoxicity in PC12 Cells

作　　者：崔婧[1] 孙宁宁 王战伟 王晶[1] 时东方[1] Cui Jing;Sun Ningning;Wang Zhanwei;Wang Jing;Shi Dongfang(Institute of Innovation Science&Technology,Central Laboratory,Changchun Normal University,Changchun 130031)

机构地区：[1]长春师范大学科技创新研究院中心实验室,长春130031

出　　处：《中国食品学报》2024年第9期114-121,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：吉林省自然科学基金项目(20230101194JC);吉林省教育厅项目(JJKH20230910KJ)。

摘　　要：目的:通过细胞实验探究茯苓酸对冈田酸(OA)诱导的PC12细胞损伤的保护作用及其机制。方法:利用40 nmol/L的OA建立PC12细胞损伤模型,以不同质量浓度茯苓酸(10,15,20μg/mL)为保护剂,检测细胞存活率,LDH释放,细胞内Ca^(2+)浓度和Caspase-3活性。利用western blot检测bax和p-taus202蛋白的表达。结果:与对照组比较,40 nmol/L OA使PC12细胞的活性降低至(64±3.6)%(P<0.01),LDH释放量增加至(141.3±3.1)%,细胞[Ca^(2+)]i浓度为(178.3±13.2)%和Caspase-3活性升高至(257.0±22.8)%(P <0.01);与模型组比较,50μg/mL茯苓酸可以使PC12细胞的存活率提高至(90.5±3.3)%(P<0.01);降低LDH释放量至(117.5±3.8)%,[Ca^(2+)]i浓度至(123.5±5.2)%和降低Caspase-3活性至(120.0±4.1)%(P<0.01)。Western blot检测结果显示,OA上调bax的表达,上调p-taus202的表达;与对照组相比,茯苓酸可以下调由OA导致的bax和p-taus202的蛋白表达上升(P<0.01)。结论:茯苓酸对OA诱导的PC12细胞具有保护作用,其机制与抑制tau蛋白的过度磷酸化有关。Objective:To investigated the protective effects of pachymic acid on okadaic acid(OA)-induced neurotoxicity in PC12 cells and identified a potential mechanism.Methods:Pachymic acid(10,15,20 μg/mL) was used as protective drugs to protect PC12 cells injured by OA(40 nmol/L).Cell viability,LDH released,intracellular Ca~(2+)concentration and caspase-3 activity were detected.The levels of bax and p-taus202 were detected by western blotting.Results:Compared with the control group,PC12 cells treated with 40 nmol/L OA was significantly decreased the viability to(64±3.6)%(P<0.01),increased the release of LDH to(141.3±3.1)% increased intracellular Ca~(2+)concentration to(123.5±5.2)% and caspase-3 activity to(257.0±22.8)%(P<0.01);compared with the model group,50 μg/mL pachymic acid significantly increased cell viability to(90.5 ±3.3)%(P <0.01),decreased LDH released to(117.5 ±3.8)%,decreased [Ca~(2+)]i,to(123.5±5.2)% and reduced caspase-3 activity to(120.0±4.1)%(P<0.01).Western blot results confirmed that OA increased the protein expression of bax and p-taus202,pachymic acid down-regulated the expression of bax and p-taus202.Conclusion:the protective effect of pachymic acid against OA-induced apoptosis in PC12 cells was related to the inhibition of tau hyperphosphorylation.

关 键 词：阿尔茨海默症 茯苓酸 PC12细胞 冈田酸 TAU蛋白 

分 类 号：TS201.4[轻工技术与工程—食品科学]